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Transgenesis by adenovirus-mediated gene transfer into mouse zona-free eggs

Nature Biotechnology volume 14, pages 982–985 (1996)Cite this article

Abstract

Zona-free mouse eggs at the pronucleus stage were infected with a replication-defective adenovirus vector containing a nuclear-targeted lacZ gene. Exogenous ß-galactosidase activity was detected in almost all eggs at the two-cell stage. Of 27 mice that developed from infected eggs, three carried the integrated exogenous gene mediated by the adenovirus. Two of the three expressed the lacZ gene, and all three mice transmitted the adenovirus-mediated transgene to F1 progeny Southern blot analysis was consistent with single copy integration. This finding should accelerate the development of new strategies for transgenesis and assist studies on the function of cloned genes in vivo.

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Author notes

  1. Tohru Tsukui

    Present address: Department ofDNA Biology and Embryo Engneering, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 108, Japan

  2. Tohru Tsukui: Corresponding author

Authors and Affiliations

  1. Department of Reproductive and Developmental Biology, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 108, Japan

    Tohru Tsukui

  2. Laboratory of Molecular Genetics, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 108, Japan

    Yumi Kanegae & Izumu Saito

  3. The Research Center for Protozoan Molecular Immunology, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, 080, Japan

    Yutaka Toyoda

Authors
  1. Tohru Tsukui
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  2. Yumi Kanegae
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  3. Izumu Saito
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  4. Yutaka Toyoda
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Tsukui, T., Kanegae, Y., Saito, I. et al. Transgenesis by adenovirus-mediated gene transfer into mouse zona-free eggs. Nat Biotechnol 14, 982–985 (1996). https://doi.org/10.1038/nbt0896-982

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  • DOI: https://doi.org/10.1038/nbt0896-982

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