Abstract
Recombinant baculoviruses are a popular means of producing heterologous protein in eukaryotic cells. Purification of recombinant proteins away from the insect cell background can, however, remain an obstacle for many developments. Recently, prokaryotic fusion protein expression systems have been developed allowing single-step purification of the heterologous protein and specific proteolytic cleavage of the affinity tag moiety from the desired antigen. Here we report the introduction of these attributes to the baculovirus system. “Baculo-GEX” vectors enable baculovirus production of fusion proteins with the above advantages, but in a eukaryotic post-translational processing environment. Glutathione-S-transferase (GST) fusions are stable cytoplasmic proteins in insect cells and may therefore be released by sonication alone, avoiding the solubility problems and detergent requirements of bacterial systems. Thus large amounts of authentic antigen may be purified in a single, non-denaturing step.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$209.00 per year
only $17.42 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Luckow, V.A. and Summers, M.D. 1988. Trends in the development of baculovinis expression vectors. Bio/Technology 6: 47–55.
Miller, L.K. 1989. Insect baculoviruses: powerful gene expression vectors. Bioessays 11: 91–95.
Uhlen, M., Nilsson, B., Guss, B., Lindberg, M., Gatenbeck, S. and Philipson, L. 1983. Gene fusion vectors based on the gene for staphylococcal protein A. Gene 23: 369–378.
Sassenfeld, H.M. and Brewer, S.J. 1984. A polypeptide fusion designed for the purification of recombinant proteins. Bio/Technology 2: 76–81.
Lilius, G., Persson, M., Bulow, L. and Mosbach, K. 1991. Metal affinity precipitation of proteins carrying genetically attached polyhistidine affinity tails. Eur. J. Biochem. 198: 499–504.
Genuine, J., Gray, J.G., Charbonneau, H., Vanaman, T. and Bastia, D. 1983. Use of gene fusions and protein-protein interaction in the isolation of a biological regulatory protein: the replication initiator protein of plasmid R6K. Proc. Natl Acad. Sci. USA 80: 6848–6852.
Riggs, P. 1992. Expression and purification of maltose-binding protein fusion, p. 16.6.1–16.6.15. In: Ausebel, F. M. et al. (Eds.). Current Protocols in Molecular Biology. Greene Publishing Associates/Wiley Interscience, New York.
Smith, D.B. and Johnson, K.S. 1988. Single-step purification of polypeptides expressed in Escherischia coli as fusion with glutathione-S-transferase. Gene 67: 31–40.
Livingstone, C. and Jones, I.M. 1989. Baculovirus expression vectors with single strand capability. Nucl. Acids Res. 17: 2366.
Matsuura, Y., Possee, R.D., Overton, H.A. and Bishop, D.H.L. 1987. Baculovirus expression vectors: the requirements for high level expression of proteins, including glycoproteins. J. Gen. Virol. 68: 1233–1250.
Martin, A.O. 1986. The purification of eukaryotic polypeptides synthesized in Escherischia coli . Biochem. J. 240: 1–12.
Kaelin, W.G. Jr., Pallas, D.C., DeCaprio, J.A., Kaye, F. and Livingstone, D.M. 1991. Identification of cellular proteins that can interact specifically with the T/E1A-binding region of the retinoblastoma gene product. Cell 64: 521–532.
Rustgi, A.K., Dyson, N. and Bernards, R. 1991. Amino-terminal domains of c-myc and N-mycproteins mediate binding to the retinoblastoma gene product. Nature 352: 541–544.
Kaelin, W.G. Jr., Krek, W., Sellers, W.R., DeCaprio, J.A., Ajchenbaum, F., Fuchs, C.S., Chittenden, T., Li, Y., Farnham, P.J., Blanar, M.A., Livingston, D.M. and Flemington, E.K. 1992. Expression cloning of a cDNA encoding a retinoblastoma-binding protein with E2F-like properties. Cell 70: 351–364.
Fikrig, E., Baithold, S.W., Kantor, F.S. and Flavell, R.A. 1990. Protection of mice against the Lyme disease agent by immunizing with recombinant OspA. Science 250: 553–556.
Johnson, K.S., Harrison, G.B.L., Lightowlers, M.W., O'Hoy, K.L., Cougle, W.G., Dempster, R.P., Lawrence, S.B., Vinton, J.G., Heath, D.D. and Rickard, M.D. 1989. Vaccination against ovine cysticercosis using a defined recombinant antigen. Nature 338: 585–587.
Hung, T., Mak, K. and Pong, K. 1990. A specificity enhancer for polymerase chain reaction. Nucl. Acids Res. 18: 4953.
King, L.A. and Possee, R.D. 1992. The Baculovirus Expression System: A Laboratory Guide. Chapman and Hall, London.
Feigner, P.L., Gadek, T.R., Holm, M., Roman, R., Chan, H.W., Wenz, M., Northrop, J.P., Ringold, G.M. and Damielsen, M. 1987. Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure. Proc. Natl Acad. Sci. USA 84: 7413–7417.
Kitts, P.A., Ayres, M.D. and Possee, R.D. 1990. Linearisation of baculovinis DNA enhances the recovery of recombinant virus expression vectors. Nucl. Acids Res. 18: 5667–5672.
Messing, J. 1983. New M13 vectors for cloning. Methods Enzymol. 101: 20–78.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Davies, A., Jowett, J. & Jones, I. Recombinant Baculovirus Vectors Expressing Glutathione–S–Transferase Fusion Proteins. Nat Biotechnol 11, 933–936 (1993). https://doi.org/10.1038/nbt0893-933
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/nbt0893-933
This article is cited by
-
Application of Baculovirus Expression Vector system (BEV) for COVID-19 diagnostics and therapeutics: a review
Journal of Genetic Engineering and Biotechnology (2022)