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Restriction Endonuclease Map of pTi Bo542, a Potential Ti Plasmid Vector for Genetic Engineering of Plants

Bio/Technology volume 2, pages 702–709 (1984)Cite this article

Abstract

We report here a physical map of pTi Bo542, a wide-host-range tumor-inducing plasmid of Agrobacterium tumefaciens. Forty-two fragments generated by the restriction endonuclease Bam HI were arranged in a circular array using electrophoretic analysis of clones containing overlapping segments of pTi Bo542. The T-DNA region (the part of the Ti plasmid transferred to the plant) was predicted by its homology to T-DNAs from heterologous octopine and nopaline Ti plasmids. Southern hybridizations against soybean tumor DNA using clones from this region as probes confirmed this prediction.

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  1. Robert T. Fraley: Monsanto Company, 800 N. Lindbergh, St. Louis, MO 63167.

  2. Mary-Dell Chilton: Ciba-Geigy Corporation, Biotechnology Research, P.O. Box 12257, Research Triangle Park, N.C. 27709

Authors and Affiliations

  1. Department of Biology, Washington University, St. Louis, MO, 63130

    Elizabeth E. Hood, George Jen, Lucy Kayes & Julie Kramer

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  1. Elizabeth E. Hood
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Hood, E., Jen, G., Kayes, L. et al. Restriction Endonuclease Map of pTi Bo542, a Potential Ti Plasmid Vector for Genetic Engineering of Plants. Nat Biotechnol 2, 702–709 (1984). https://doi.org/10.1038/nbt0884-702

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