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Phage display extends its reach

Nature Biotechnology volume 24, pages 793–794 (2006)Cite this article

Proteins that could not be displayed on the surface of phage are now amenable to this powerful selection technology.

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Figure 1: Comparison of the commonly used Sec signal sequence and the SRP signal sequence for phage display of proteins fused to the phage minor coat protein p3.

Bob Crimi

References

  1. Steiner, D., Forrer, P., Stumpp, M.T. & Plückthun, A. Nat. Biotechnol. 24, 823–831 (2006).

    Article  CAS  Google Scholar 

  2. Blobel, G. & Dobberstein, B. J. Cell Biol. 67, 835–851 (1975).

    Article  CAS  Google Scholar 

  3. Takeishi, K., Yasumura, M., Pirtle, R. & Inouye, M. J. Biol. Chem. 251, 6259–6266 (1976).

    CAS  PubMed  Google Scholar 

  4. Inouye, H. & Beckwith, J. Proc. Natl. Acad. Sci. USA 74, 1440–1444 (1977).

    Article  CAS  Google Scholar 

  5. Huber, D. et al. J. Bacteriol. 187, 2983–2991 (2005).

    Article  CAS  Google Scholar 

  6. Huber, D. et al. Proc. Natl. Acad. Sci. USA 102, 18872–18877 (2005).

    Article  CAS  Google Scholar 

  7. Schierle, C.F. et al. J. Bacteriol. 185, 5706–5713 (2003).

    Article  CAS  Google Scholar 

  8. Sone, M., Kishigami, S., Yoshihisa, T. & Ito, K. J. Biol. Chem. 272, 6174–6178 (1997).

    Article  CAS  Google Scholar 

  9. Kumamoto, C.A. & Gannon, P.M. J. Biol. Chem. 263, 11554–11558 (1988).

    CAS  PubMed  Google Scholar 

  10. Li, P., Beckwith, J. & Inouye, H. Proc. Natl. Acad. Sci. USA 85, 7685–7689 (1988).

    Article  CAS  Google Scholar 

  11. Andersson, H. & von Heijne, G. Embo. J. 12, 683–691 (1993).

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Ave., Boston, 02115, Massachusetts, USA

    Damon Huber & Jonathan Beckwith

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  1. Damon Huber
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  2. Jonathan Beckwith
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Huber, D., Beckwith, J. Phage display extends its reach. Nat Biotechnol 24, 793–794 (2006). https://doi.org/10.1038/nbt0706-793

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