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Single Tree Genetic Linkage Mapping in Conifers Using Haploid DNA from Megagametophytes

Bio/Technology volume 10, pages 686–690 (1992)Cite this article

Abstract

We report a unique application of the Random Amplified Polymorphic DNA (RAPD) technique for genetic linkage mapping of a single spruce tree using haploid DNA from megagametophyte tissue of individual seeds. Sixty–one segregating loci were analysed for reproducibility, inheritance and linkage. Forty–seven of the 61 markers were distributed into 12 linkage groups and covered 873.8 cM. The 14 markers not associated with any linkage group should be assigned to linkage groups as more markers are added to the map. This new approach quickly provides molecular genetic markers that are simple to evaluate for constructing genetic linkage maps and for other related genetic studies in forest tree species.

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Author information

Author notes

  1. Lomas K. Tulsieram

    Present address: Allelix Crop Technologies, Inc., 6850 Goreway Drive, Mississauga, Ontario, Canada, L4V 1P1

  2. John E. Carlson: Corresponding author.

Authors and Affiliations

  1. Biotechnology Laboratory, University of British Columbia, 237 Wesbrook Building, 6174 University Boulevard, Vancouver, British Columbia, Canada, V6T 1W5

    Lomas K. Tulsieram, Jeffrey C. Glaubitz & John E. Carlson

  2. British Columbia Forest Service, Kalamalka Research Station, Vernon, British Columbia, Canada, V1B 2C7

    Gyula Kiss

Authors
  1. Lomas K. Tulsieram
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  2. Jeffrey C. Glaubitz
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  3. Gyula Kiss
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  4. John E. Carlson
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Tulsieram, L., Glaubitz, J., Kiss, G. et al. Single Tree Genetic Linkage Mapping in Conifers Using Haploid DNA from Megagametophytes. Nat Biotechnol 10, 686–690 (1992). https://doi.org/10.1038/nbt0692-686

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  • DOI: https://doi.org/10.1038/nbt0692-686

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