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Choosing a Host Cell for Active Recombinant Factor VIII Production Using Vaccinia Virus

Bio/Technology volume 5, pages 389–392 (1987)Cite this article

Abstract

The recent molecular cloning of factor VIII (FVIII or antihaemophilic factor)1–3 has brought new hope to haemophilia A patients of a safer therapeutic preparation. The choice of a host cell for the expression of such a large and unstable protein4 in a biologically active form is limited however because of the time consuming process of screening cell lines. Having cloned the FVIII cDNA, we have used the vaccinia virus (VV) expression system5,6 in order to screen rapidly several different cell lines for active recombinant FVIII production. We show here that the ability (or inability) to express active FVIII is dependent on the host cell used. Baby hamster kidney 21 (BHK21) cells have given the most encouraging results so far. The stabilizing effect of von Willebrand factor (vWf) on the recombinant FVIII when added to the culture medium is also demonstrated.

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Author information

Author notes

  1. Marie-Louise Wiesel and Jean-Pierre Cazenave: Service d'Hémostase et de Thrombose, Centre Régional de Transfusion Sanguine, 10 Rue Spielmann, 67085 Strasbourg Cedex, France.

  2. Claudine Mazurier: Laboratoire d'Hémostase, Centre Régional de Transfusion Sanguine, 19–21 Rue Camille-Guérin, 59012 Lille Cedex, France.

  3. Hendrik Van de Pol: Département de Recherche et Développement, Centre National de Transfusion Sanguine, Avenue des Tropiques, B.P. 100, 91943 Les Ulis Cedex, France.

  4. Yves Piquet: Centre Régional de Transfusion Sanguine, P. Amélie-Raba-Léon, B.P. 24, 33035 Bordeaux Cedex, France.

Authors and Affiliations

  1. TRANSGENE S.A., 11 Rue de Molsheim, 67000, Strasbourg, France

    Andrea Pavirani, Pierre Meulien, Huguette Harrer, Fabienne Schamber, Karin Dott, Dominique Villeval, Yves Cordier & Jean-Pierre Lecocq

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  1. Andrea Pavirani
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  2. Pierre Meulien
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  3. Huguette Harrer
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  4. Fabienne Schamber
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  8. Marie-Louise Wiesel
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  10. Hendrik Van de Pol
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  12. Jean-Pierre Cazenave
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  13. Jean-Pierre Lecocq
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Pavirani, A., Meulien, P., Harrer, H. et al. Choosing a Host Cell for Active Recombinant Factor VIII Production Using Vaccinia Virus. Nat Biotechnol 5, 389–392 (1987). https://doi.org/10.1038/nbt0487-389

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