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Cloning and Expression in Escherichia Coli of the Bovine Papillomavirus L1 and L2 Open Reading Frames

Bio/Technology volume 2, pages 356–360 (1984)Cite this article

Abstract

The two large open reading frames, LI and L2, in the nontransforming region of the bovine papilloma virus type 1 (BPV-1) genome were cloned and expressed in Escherichia coli. Each was constructed to be expressed in two forms: a naturally terminating protein fused to a non-BPV leader peptide and part of a larger fusion protein terminating with E. coli β-galactosidase (β-gal). Anti-BPV-1 sera identified a BPV-1-specific product for the naturally terminating LI, but not the L2, construction. Antisera generated against the β-gal fusions of both LI and L2 reacted with purified BPV-1, and this antisera neutralized purified virions, which prevented BPV-1 transformation of mouse C127 cells.

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Author notes

  1. Donald L. Glassman: College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108

Authors and Affiliations

  1. Molecular Genetics, Inc., Minnetonka, MN, 55343

    William P. Pilacinski, Richard A. Krzyzek, Peter L. Sadowski & Alan K. Robbins

Authors
  1. William P. Pilacinski
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  2. Donald L. Glassman
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  3. Richard A. Krzyzek
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  4. Peter L. Sadowski
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  5. Alan K. Robbins
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Pilacinski, W., Glassman, D., Krzyzek, R. et al. Cloning and Expression in Escherichia Coli of the Bovine Papillomavirus L1 and L2 Open Reading Frames. Nat Biotechnol 2, 356–360 (1984). https://doi.org/10.1038/nbt0484-356

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