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Cloning and Expression in Streptomyces lividans of Clustered Erythromycin Biosynthesis Genes from Streptomyces erythreus

Bio/Technology volume 4, pages 229–232 (1986)Cite this article

Abstract

Erythromycin is a clinically important antibiotic produced by Streptomyces erythreus. We cloned DNA from S. erythreus in a cosmid vector bifunctional for E. coli and Streptomyces and identified an E. coli clone containing erythromycin biosynthetic genes by hybridization to a cloned erythromycin resistance gene from S. erythreus. A plasmid, pKC488, was isolated and introduced into Streptomyces lividans. Transformants produced an antibiotic indistinguishable from erythromycin A.

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Authors and Affiliations

  1. Department of Molecular Genetics, Lilly Research Laboratories, Indianapolis, Indiana, 46285

    R. Stanzak, P. Matsushima, R. H. Baltz & R. N. Rao

Authors
  1. R. Stanzak
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  2. P. Matsushima
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  3. R. H. Baltz
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  4. R. N. Rao
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Stanzak, R., Matsushima, P., Baltz, R. et al. Cloning and Expression in Streptomyces lividans of Clustered Erythromycin Biosynthesis Genes from Streptomyces erythreus. Nat Biotechnol 4, 229–232 (1986). https://doi.org/10.1038/nbt0386-229

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  • DOI: https://doi.org/10.1038/nbt0386-229

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