Peggy S. Eis, Marilyn C. Olson, Tsetska Takova, Michelle L. Curtis, Sarah M. Olson, Tatiana I. Vener, Hon S. Ip, Kevin L. Vedvik, Christian T. Bartholomay, Hatim T. Allawi, Wu-Po Ma, Jeff G. Hall, Michelle D. Morin, Tom H. Rushmore, Victor I. Lyamichev, and Robert W. Kwiatkowski.
Nat. Biotechnol. 19, 673–676 (2001).
Because of a proofreading error, the lysis buffer mentioned in the Figure 4 legend was described incorrectly as containing “200 mg/ml tRNA”.
The correct sentence is as follows:
Cell lysate preparation: 40 μl lysis buffer (20 mM Tris, pH 8, 5 mM MgCl2, 0.5% NP-40, 20 μg/ml tRNA) was added per well, cells were lysed at room temperature for 3–5 min, 30 μl of each lysate sample were transferred to a 96-well microplate and cellular nucleases inactivated by heating the lysates for 15 min at 75–80°C.
We regret the error.
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The online version of the original article can be found at 10.1038/90290
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Erratum: An invasive cleavage assay for direct quantitation of specific RNAs . Nat Biotechnol 20, 307 (2002). https://doi.org/10.1038/nbt0302-307
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DOI: https://doi.org/10.1038/nbt0302-307
