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Pseudomonas Aeruginosa Secretes and Correctly Processes Human Growth Hormone

Bio/Technology volume 2, pages 161–165 (1984)Cite this article

Abstract

Pseudomonas aeruginosa transformed with a plasmid containing a gene encoding the mature form of human growth hormone (hGH) preceded by an ATG codon to initiate translation expresses methionyl-hGH its cytoplasm. hGH produced in P. aeruginosa cells transformed with a plasmid containing a gene for the natural hGH precursor is transported across the inner membrane and has the NH2-terminal amino acid sequence of authentic mature hGH.

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Authors and Affiliations

  1. Genentech, Inc., 460 Point San Bruno Boulevard, South San Francisco, CA, 94080

    Gregory L. Gray, Kathleen A. McKeown, Andrew J. S. Jones, Peter H. Seeburg & Herbert L. Heyneker

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  1. Gregory L. Gray
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  2. Kathleen A. McKeown
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  4. Peter H. Seeburg
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  5. Herbert L. Heyneker
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Gray, G., McKeown, K., Jones, A. et al. Pseudomonas Aeruginosa Secretes and Correctly Processes Human Growth Hormone. Nat Biotechnol 2, 161–165 (1984). https://doi.org/10.1038/nbt0284-161

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