Removal of antibiotic resistance genes from transgenic tobacco plastids

Siriluck Iamtham and Anil Day Nat. Biotechnol. 18, 1172–1176 (2000)

Because of a production error the values of several compounds were incorrectly noted in the Experimental Protocol under “Generation and analysis of transplastomic plants”. The corrected values are given below. We regret the error.

Shoots and green cell lines were selected on spectinomycin dihydrochloride pentahydrate (Duchefa, Haarlem, The Netherlands) plus streptomycin sulfate (Sigma, St. Louis, MO), each at 500 μg/ml. Clones were transferred to RMOP medium containing 5 μg/ml glufosinate-ammonium (Dr. Ehrenstorfer GmbH, Augsburg, Germany) after 9–34 weeks for a second cycle of regeneration. . . .The 42 herbicide-resistant clones were rooted on MS medium containing 1 μg/ml glufosinate-ammonium and transferred to soil.