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Imaging without the blur

Deconvolution microscopy

Nature Cell Biology volume 11, pages S16–S17 (2009)Cite this article

By the 1980s, fluorescent probes for many specific cellular molecules were available. However, visualizing their distribution in relatively thick specimens was tricky. Researchers hoped to reconstruct 3D images from stacks of 'optical sections' — images captured at consecutive focal planes through a thick object. However, interpreting the information in any given focal plane was complicated by the presence of out-of-focus fluorescence from neighbouring planes.

These papers ... demonstrated the high level of 3D macromolecular structure present in cells and the structural complexity of the cell interior. Jason Swedlow

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References

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Authors and Affiliations

  1. Senior Editor, Nature Reviews Neuroscience,

    Katherine Whalley

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  1. Katherine Whalley
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Whalley, K. Imaging without the blur. Nat Cell Biol 11 (Suppl 1), S16–S17 (2009). https://doi.org/10.1038/ncb1949

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