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Diagnosis: Amyloidosis

Lab Animal volume 32, pages 24–25 (2003)Cite this article

We saw microscopic evidence of a systemic amyloidosis in most organs. Amyloid deposits in hematoxylin- and eosin-stained tissue sections appeared as pale, amorphous eosinophilic deposits, homogenous-to-fibrillar material. When stained with Congo Red and examined under polarized light, the amyloid had an apple green appearance. Amyloid infiltrates and deposits diffusely effaced, replaced, or compressed 80% of the hepatocytes examined in liver sections. The amyloid destroyed the hepatic plate architecture, and severely congested the remaining patent sinusoids.

Ulcerative, focally extensive, chronic-active, marked colitis with amyloid was present in the large intestine. Approxi-mately 25% of the circumference of the mucosal epithelium was ulcerated. The lamina propria in the affected area was markedly expanded by fibrous connective tissue proliferation, neovascularization, and infiltration of amyloid that effaced pre-existing colonic tissue. Inflammatory infiltrates consisted primarily of lymphocytes, plasma cells, neutrophils, and eosinophils. Sections of lymph nodes had deposits of amyloid in lymphoid follicles and were depleted of mature lymphocytes. Moderate erythrophagocytosis was present in lymph node medullary areas. Other organs were amyloid-free and appeared essentially normal.

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Authors and Affiliations

  1. Goldstein, Baptiste, Arif, Smith, Coplan, and Scharf are affiliated with SUNY Downstate Medical Center, Brooklyn, NY.,

    Max Goldstein BS, Shirn Baptiste BS, Muhammad Arif MD,  Eric Smith &  Jeremy Coplan

  2. Ragland is affiliated with BioReliance, Rockville, MD.,

    Daniel Ragland DVM

  3. SUNY Downstate Medical Center, 450 Clarkson Ave., Box 47, Brooklyn, NY 11203-2098,

    Bruce Scharf DVM

Authors
  1. Max Goldstein BS
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  2. Shirn Baptiste BS
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  3. Muhammad Arif MD
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  6. Daniel Ragland DVM
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  7. Bruce Scharf DVM
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Correspondence to Bruce Scharf DVM.

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Please send reprint requests to Scharf at SUNY Downstate Medical Center, 450 Clarkson Ave., Box 47, Brooklyn, NY 11203-2098

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Goldstein, M., Baptiste, S., Arif, M. et al. Diagnosis: Amyloidosis. Lab Anim 32, 24–25 (2003). https://doi.org/10.1038/laban0603-24

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