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Lack of association between α-adducin G460W polymorphism and hypertension: evidence from a case–control study and a meta-analysis

Journal of Human Hypertension volume 24, pages 467–474 (2010)Cite this article

Abstract

The past two decades have seen an upsurge in detecting the genetic determinants of hypertension. Thereafter, α-adducin gene ranks high and one polymorphism, G460W (rs4961), has attracted special attention. We first performed a case–control study to investigate the association of this polymorphism with essential hypertension among Shanghai residents, and then meta-analyzed all available evidence. A total of 950 subjects were recruited for genetic association study. Genotyping for G460W was conducted using PCR and restriction fragment length polymorphism techniques. Meta-analysis search was limited to articles published in English and studies on humans. Data and study quality were assessed in duplicate. Publication bias was evaluated using the fail-safe number. Our case–control study provided no evidence for the association of G460W with essential hypertension, even under assumptions of three genetic modes of inheritance (P>0.05). The subsequent meta-analysis including 15 studies with 4417 cases and 5716 controls also failed to demonstrate overall this association, even upon stratification by race (Caucasians and Asians). For example, the summary odds ratio (OR) under a random effects model indicated that carriers of 460W allele were 1.09 times more likely to develop hypertension (95% confidence interval (CI), 0.96–1.24; P=0.19) among Asians, whereas a protective effect of this allele was observed in Caucasians (OR, 0.93; 95% CI, 0.73–1.18; P=0.54). The fail-safe number at the level of 0.05 was in favour of our findings. Our case–control study and the following meta-analysis failed to provide evidence for the genetic association of α-adducin gene G460W polymorphism with hypertension.

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Acknowledgements

We acknowledge the assistance and cooperation of the faculty and staff of the Department of Hypertension at Ruijin Hospital. We thank the dedicators for their enthusiastic participation. This work was financially supported by the Shanghai ‘Chen Guang’ Project (09CG12); the Natural Science Foundation of Shanghai (09ZR1426200); two Excellent Young Teachers Programs, one from Ruijin Hospital (WN) and the other from Shanghai City (WN); the Science Fund of Shanghai Jiaotong University School of Medicine (09XJ21019) and the National Science Foundation for Young Scientists of China (Grant number 30900808).

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Authors and Affiliations

  1. State Key Laboratory of Medical Genomics, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, PR China

    W-Q Niu, Y Zhang, K-D Ji, P-J Gao & D-L Zhu

  2. Shanghai Institute of Hypertension, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, PR China

    W-Q Niu, Y Zhang, K-D Ji, P-J Gao & D-L Zhu

  3. Sino-French Research Center for Life Science and Genomics, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, PR China

    W-Q Niu, Y Zhang, K-D Ji & D-L Zhu

  4. Shanghai Key Laboratory of Vascular Biology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, PR China

    K-D Ji & P-J Gao

  5. Laboratory of Vascular Biology, Institute of Health Science Center, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai, PR China

    P-J Gao

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Correspondence to D-L Zhu.

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Niu, WQ., Zhang, Y., Ji, KD. et al. Lack of association between α-adducin G460W polymorphism and hypertension: evidence from a case–control study and a meta-analysis. J Hum Hypertens 24, 467–474 (2010). https://doi.org/10.1038/jhh.2009.88

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