Summary
PCR-based genotyping of MNSs blood group system was investigated in combination with restriction fragment length polymorphism (RFLP), single-strand conformation polymorphism (SSCP) and allele-specific PCR amplification (ASPA) techniques. M and N alleles are based on three nucleotide substitutions in exon 2 and one base change (G or T) in an intron of glycophorin A locus. The latter single base change was also found among M alleles analyzed in this study, so that M allele appeared to be subdivided into MG and MT. All three alleles, MG, MT and N were identified clearly by RFLP or SSCP analysis following a single amplification. S and s alleles are based on one nucleotide substitution in exon 3 of glycophorin B gene. Genotyping of Ss blood group system was also explored by PCR-SSCP or ASPA analysis, and problems in the methods were discussed.
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Akane, A., Kobayashi, T., Li, ZX. et al. PCR-based genotyping of MNSs blood group: Subtyping of M allele to MG and MT . Jap J Human Genet 42, 489–498 (1997). https://doi.org/10.1007/BF02767025
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DOI: https://doi.org/10.1007/BF02767025
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