Summary
TBP (TATA box-binding protein) participates in the expression of eukaryotic genes transcribed by RNA polymerases I, II, and III. Molecular cloning of human TBP revealed that the N-terminal region contains a polymorphic (CAG)n repeat. We report here the direct localization of human TBP gene to chromosome 6q2705→qter region by fluorescencein situ hybridization, using the cDNA clone with or without the (CAG)n repeat as a probe.
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Bollag RJ, Siegfried Z, Cebra-Thomas JA, Garvey N, Davison EM, Silver LM (1994): An ancient family of embryonically expressed mouse genes sharing a conserved protein motif with the T locus. Nature Genet7: 383–389
Herrmann BG, Labeit S, Poustka A, King TR, Lehrach H (1990): Cloning of the T gene required in mesoderm formation in the mouse. Nature343: 617–622
Hernandez N (1993): TBP, a universal eukaryotic transcription factor. Genes Develop4: 1291–1308
HGM11 (1911): Eleventh International Workshop on Human Gene Mapping. Cytogenet Cell Genet58: 1–2200
Hoffmann A, Sinn E, Yamamoto T, Wang J, Roy A, Horikoshi M, Roeder RG (1990): Highly conserved core domain and unique N terminus with presumptive regulatory motifs in a human TATA factor (TFIID). Nature346: 387–390
Hori T, Takahashi E, Ayusawa D, Takeishi K, Kaneda S, Seno T (1990): Regional assignment of the human thymidylate synthase (TS) gene to chromosome band 18q11.32 by nonisotopicin situ hybridization. Hum Genet85: 576–580
Imbert G, Trottier Y, Beckmann J, Mandel JL (1994): The gene for the TATA binding protein (TBP) that contains a highly polymorphic protein coding CAG repeat maps to 6q27. Genomics21: 667–668
Kispert A, Herrmann BG (1993): TheBrachyury gene encodes a novel DNA binding protein. EMBO J12: 3211–3220
Koide R, Ikeuchi T, Onodera O, Tanaka H, Igarashi S, Endo K, Takahashi H, Kondo R, Ishikawa A, Hayashi T, Saito M, Tomoda A, Miike T, Naito H, Ikuta F, Tsuji S (1994): Unstable expansion of CAG repeat in hereditary dentatorubral-pallidoluysian atrophy (DRPLA). Nature Genet6: 9–13
Nagafuchi S, Yanagisawa H, Sato K, Shirayama T, Ohsaki E, Bundo M, Takeda T, Tadokoro K, Kondo I, Murayama N, Tanaka Y, Kikushima H, Umino K, Kurosawa H, Furukawa T, Nihei K, Inoue T, Sano A, Komure O, Takahashi M, Yoshizawa T, Kanazawa I, Yamada M (1994): Dentatorubral and pallidoluysian atrophy expansion of an unstable CAG trinucleotide on chromosome 12p. Nature Genet6: 14–18
O'Brien SJ (eds) (1993): Human maps. In: Genetic maps, 6th edn., Cold Spring Harbor Press, Cold Spring Harbor, New York
Polymeropoulos MH, Rath DS, Xiao H, Merril CR (1991): Trinucleotide repeat polymorphism at the human transcription factor IID gene. Nucleic Acids Res19: 4307
Saito F, Sasaki S, Chepelinsky AB, Fushimi K, Marumo F, Ikeuchi T (1995): Human AQP2 and MIP genes, two members of the MIP family, map within chromosome band 12q13 on the basis of two-color FISH. Cytogenet Cell Genet68: 45–48
Sutherland GR, Richards RI (1993): Dynamic mutations on the move. J Med Genet30: 978–981
Takahashi E, Yamanouchi M, Tsuji H, Hitomi A, Meuth M, Hori T (1991): Chromosome mapping of the human cytidine-5′-triphosphate synthetase (CTPS) gene to band 1p34.1-p34.3 by fluorescencein situ hybridization. Hum Genet88: 119–121
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Saito, F., Yamamoto, T., Horikoshi, M. et al. Direct mapping of the human TATA box-binding protein (TBP) gene to 6q27 by fluorescencein situ hybridization. Jap J Human Genet 39, 421–425 (1994). https://doi.org/10.1007/BF01892387
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DOI: https://doi.org/10.1007/BF01892387