Summary
We have developed a one-step polymerase chain reaction (PCR) technique which detects a sequence on the human Y chromosome and an autosomal sequence in one reaction. The method is very reliable for the sex determination, as the detection of the autosome-specific signal ensures the presence of DNA in the specimen even in the absence of the Y-specific signal.
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References
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Iida, T., Nakahori, Y., Tanaka, K. et al. Sex identification by polymerase chain reaction using a Y-autosome homologous primer set. Jap J Human Genet 38, 429–431 (1993). https://doi.org/10.1007/BF01907990
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DOI: https://doi.org/10.1007/BF01907990