Summary
The genetic markers of a Korean woman and her allegedly adopted child were compared to test her maternity. None of 21 conventional markers, including 8 red cell antigens, 6 red cell enzymes, and 7 serum proteins, excluded the maternity. These results indicate a maternal probability of 0.31 which was too low to conclude the true maternity. Human leukocyte antigen (HLA)-A,-B,-C and-DR haplotyping was also uninformative in this case. The maternity was consequently excluded by the observation of a difference in 2 of 5 variable number of tandem repeat (VNTR) markers.
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Abbreviations
- PGM1:
-
phosphoglucomutase 1
- ACP1:
-
acid phosphatase 1
- ESD:
-
esterase D
- GPT:
-
soluble glutamic-pyruvate transaminase
- PGD:
-
phosphogluconate dehydrogenase
- GLO1:
-
glyoxalase 1
- HP:
-
haptoglobin
- GC:
-
group-specific component
- TF:
-
transferrin
- PI:
-
α1-antitrypsin
- AHSG:
-
α2HS-glycoprotein
- Gm:
-
H chain of ψ-globulin
- Km:
-
L chain of ψ-globulin
References
Akane, A., Matsubara, K., Shiono, H., Yuasa, I., Yokota, S., Yamada, M. and Nakagome, Y. 1990. Paternity testing: blood group systems and DNA analysis by VNTR markers.J. Forensic Sci. 35: 1217–1225.
Jeffreys, A.J., Royle, N.J., Wilson, V. and Wong, Z. 1988. Spontaneous mutation rates to new length alleles at tandem-repetitive hypervariable loci in human DNA.Nature 332: 278–281.
Nakamura, Y., Leppert, M., O'Connell, P., Wolff, R., Holm, T., Culver, M., Martin, C., Fujimoto, E., Hoff, M., Kumkin, E. and White, R. 1987a. Variable number of tandem repeat (VNTR) markers for human gene mapping.Science 235: 1616–1622.
Nakamura, Y., Gillilan, S., O'Connel, P., Leppert, M., Lathrop, G.M., Lalouel, J.-M. and White, R. 1987b. Isolation and mapping of a polymorphic DNA sequence pYNH24 on chromosome 2 (D2S44).Nucleic Acids Res. 15: 10073.
Nakamura, Y., Fujimoto, E., O'Connel, P., Leppert, M., Lathrop, G.M., Lalouel, J.-M. and White, R. 1987c. Isolation and mapping of a polymorphic DNA sequence (pEFD126.3) on chromosome 9q (D9S7).Nucleic Acids Res. 15: 10607.
Nakamura, Y., Holm, T., Gillilan, S., Leppert, M., O'Connel, P., Lathrop, G.M., Lalouel, J.-M. and White, R. 1988a. Isolation and mapping of a polymorphic sequence (pTHH59) on chromosome 17q (D17S4).Nucleic Acids Res. 16: 3598.
Nakamura, Y., Culver, M., Sergeant, L., Leppert, M., O'Connel, P., Lathrop, G.M., Lalouel, J.-M. and White, R. 1988b. Isolation and mapping of a polymorphic DNA sequence (pYNZ2) on chromosome 1p (D1S57).Nucleic Acids Res. 16: 4747.
Wolff, R.K., Nakamura, Y. and White, R. 1988. Molecular characterization of a spontaneously generated new allele at a VNTR locus: No exchange of flanking DNA sequences.Genomics 3: 247–351.
Yokoi, T., Nata, M., Odaira, T. and Sagisaka, K. 1990. Hypervariable polymorphic VNTR loci for parentage testing and individual identification.Jpn. J. Human Genet. 35: 179–188.
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Akane, A., Nakajima, H., Shiono, H. et al. A case of maternity testing: Exclusion by polymorphic VNTR markers of DNA. Jap J Human Genet 35, 319–323 (1990). https://doi.org/10.1007/BF01883754
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DOI: https://doi.org/10.1007/BF01883754
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