Summary
A permanent fibroblast cell line of Cockayne syndrome (CS) belonging to complementation group B (CS1BESV) was established by simian virus 40 (SV40) transformation. The ultraviolet light (UV)-sensitivity of CS1BESV was similar to that of parental primary CS1BE fibroblast. The recovery of the rate of semiconservative DNA synthesis after UV-irradiation (12 J/m2) was absent in CS1BESV. With respect to the rate of semiconservative DNA synthesis after UV-irradiation (12 J/m2), CS1BESV complemented the primary fibroblast of group A CS (CS3BE) but not group B CS (CS1BE), confirming that CS1BESV belongs to complementation group B. The CS1BESV cells were transfected with recombinant vectors (pSV2gpt) carrying theEscherichia coli xanthine-guanine phosphoribosyltransferase gene (Ecogpt). They exhibited stable transformation frequency of 1×10−3. The CS1BESV cells will be useful as a permanent source of supply of parental group B CS cells for genetic complementation tests of CS and as a DNA transfer recipient for cloning of the gene which is deficient in CS.
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Andrews, A.D., Barrett, S.F., Yoder, F.W., and Robbins, J.H. 1978. Cockayne's syndrome fibroblasts have increased sensitivity to ultraviolet light but normal rates of unscheduled DNA synthesis.J. Invest. Dermatol. 70: 237–239.
Bishop, J.M. 1983. Cancer genes come of age.Cell 32: 1018–1020.
Cleaver, J.E. 1982. Normal reconstruction of DNA supercoiling and chromatin structure in Cockayne syndrome cells during repair of damage from ultraviolet light.Am. J. Hum. Genet. 34: 566–575.
Cooper, G.M. 1982. Cellular transforming genes.Science 218: 801–806.
Gorman, C., Padmanabhan, R., and Howard, B.H. 1983. High efficiency DNA mediated transformation of primate cells.Science 221: 551–553.
Hoar, D.I. and Waghorne, C. 1978. DNA repair in Cockayne syndrome.Am. J. Hum. Genet. 30: 590–601.
Ikenaga, M., Inoue, M., Kozuka, T., and Sugita, T. 1981. The recovery of colony forming ability and the rate of semi-conservative DNA synthesis in ultraviolet-irradiated Cockayne and normal human cells.Mutat. Res. 37: 87–91.
Land, H., Parada, L.F., and Weinberg, R.A. 1983. Cellular oncogenes and multistep carcinogenesis.Science 222: 771–778.
Lehmann, A.R., Kirk-Bell, S., and Mayne, L.V. 1979. Abnormal kinetics of DNA synthesis in ultraviolet light-irradiated cells from patients with Cockayne syndrome.Cancer Res. 39: 4237–4241.
Lehmann, A.R. 1982. Three complementation groups in Cockayne syndrome.Mutat. Res. 106: 347–356.
MacInnes, M.A., Bingham, J.M., Thompson, L.H., and Strniste, G.F. 1984. DNA-mediated cotransfer of excision repair capacity and drug resistance into Chinese hamster ovary mutant cell line UV-135.Mol. Cell. Biol. 4: 1152–1158.
Mayne, L.V. and Lehmann, A.R. 1982. Failure of RNA synthesis to recover after UV irradiation: An early defects in cells from individuals with Cockayne's syndrome and xeroderma pigmentosum.Cancer Res. 42: 1473–1478.
Okada, Y. and Murayama, F. 1966. Requirement of calcium ions for the cell fusion reaction of animal cells by HVJ.Exp. Cell Res. 44: 527–551.
Rubin, J.S., Joyner, A.L., Bernstein, A., and Whitmore, G.F. 1983. Molecular identification of a human DNA repair gene following DNA-mediated gene transfer.Nature 306: 206–208.
Schmickel, R.D., Chu, E.H.Y., Trosko, J.E., and Chang, C.C. 1977. Cockayne syndrome: A cellular sensitivity to ultraviolet light.Pediatrics 60: 135–139.
Tanaka, K., Kawai, K., Kumahara, Y., Ikenaga, M., and Okada, Y. 1981. Genetic complementation groups in Cockayne syndrome.Somat. Cell Genet. 7: 445–455.
Weinberg, R.A. 1982. Fewer and fewer oncogenes.Cell 30: 3–4.
Westerveld, A., Hoeijmakers, J.H.J., van Duin, M., de Wit, J., Odijk, H., Pastink, A., Wood, R.D., and Bootsma, D. 1984. Molecular cloning of a human DNA repair gene.Nature 310: 425–429.
Wigler, M., Pellicer, A., Silverstein, S., and Axel, R. 1978. Biochemical transfer of single-copy eukaryotic genes using total cellular DNA as donor.Cell 14: 725–731.
Yagi, T. and Takebe, H. 1983. Establishment by SV40 transformation and characteristics of a cell line of xeroderma pigmentosum belonging to complementation group F.Mutat. Res. 112: 59–66.
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Tanaka, K., Kawai, K., Kumahara, Y. et al. Establishment of Cockayne syndrome fibroblast cell line belonging to complementation group B by SV40 transformation. Jap J Human Genet 30, 21–29 (1985). https://doi.org/10.1007/BF01883670
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DOI: https://doi.org/10.1007/BF01883670