Summary
HLA-DRw9 antigen, of which frequency is relatively high in Japanese, is identified and analyzed by two-dimensional gel electrophoresis using anti-DRw9 alloantisera and monoclonal antibodies against human class II antigens. The DRw9 antigen consists of one set of heavy and light chains on the cell surface and the Ii chain is associated with the heavy chain-light chain complex in intracellular regions. The DRw9 light chain is distinct from the DR4 and DR7 light chains but the DRw9 heavy chain is identical with the DR4 and DR7 heavy chain, suggesting that the DRw9 light chain carries the antigenic specificity recognized by the anti-DRw9 alloantisera. The amount of the DRw9 antigen is extremely large compared with the other class II antigens in the total class II antigen pool, as reported previously for the DR4 antigen. The data presented in this paper indicate that the DRw9 antigen is present as an independent DR molecule and has the polypeptide constitution characteristic of the DR antigen.
Similar content being viewed by others
Article PDF
References
Baur, M.P., and Danilovs, J.A. 1981. Population analysis of HLA-A, B, C, DR, and other genetic markers. InHistocompatibility Testing 1980, Terasaki, P.J., ed., UCLA Press, Los Angeles, pp. 955–993.
Bonner, W.M., and Laskey, R.A. 1974. A film detection method for tritium-labeled proteins and nucleic acids in polyacrylamide gels.Eur. J. Biochem. 46: 83–88.
Brickell, P.M., McConnell, I., Milstein, C., and Wright, B. 1981. A monoclonal antibody to the HLA-DR product recognizes a polymorphic Ia determinant in mice.Immunology 43: 493–501.
Colombe, B., and Payne, R. 1981. 8th Workshop Joint Reports 8w12 (DRw9+10). InHistocompatibility Testing 1980, Terasaki, P.J., ed., UCLA Press, Los Angeles, pp. 543–545.
Charron, D.J., and McDevitt, H.O. 1980. Characterization of HLA-D-region antigens by two-dimensional gel electrophoresis. Molecular genotyping.J. Exp. Med. 152: 18s-36s.
De Kretser, T.A., Crumpton, M.J., Bodmer, J.G., and Bodmer, W.F. 1982. Demonstration of two distinct light chains in HLA-DR-associated antigens by two-dimensional gel electrophoresis.Eur. J. Immunol. 12: 214–221.
Geha, R.S., Milgrom, H., Broff, M., Aipert, S., Martin, S., and Yunis, E.J. 1979. Effect of anti-HLA antisera on macrophage-T cell interaction.Proc. Natl. Acad. Sci. U.S.A. 76: 4038–4041.
HLA Nomenclature Committee. 1980. Nomenclature for factors of the HLA system, 1980.Tissue antigen 16: 113–117.
Hurley, C.K., Geiles, R.C., and Capra, J.D. 1983. The human MHC: evidence for multiple HLA-D-region genes.Immunol. Today 4: 219–226.
Jones, P.P. 1980. Analysis of radiolabeled lymphocyte proteins by one- and two-dimensional polyacrylamide gel electrophoresis. InSelected Methods in Cellular Immunology, Shigi, S.M., ed., W.H. Freeman and Company, San Francisco, pp. 398–440.
Koyama, K., Nakamuro, K., Tanigaki, N., and Pressman, D. 1977. Alloantigens of human lymphoid cell lines; ‘Human Ia-like antigens’: Alloantigenic activity and cell line, organ and tissue distribution as determined by radioimmunoassay.Immunology 33: 217–230.
Lampson, L.A., and Levy R. 1980. Two populations of Ia-like molecules on a human B cell line.J. Immunol. 125: 292–299.
Molner, J., Markovic, G., Chao, H., and Molnar, Z. 1969. Glycoproteins of Ehrlich ascites carcinoma cells: Separation of plasma and endoplasmic reticular membrane fractions.Arch. Biochem. Biophys. 134: 524–532.
Owen, M., Kissonerghis, A.M., Lodish, H.F., and Crumpton, M.J. 1981. Biosynthesis and maturation of HLA-DR antigensin vivo.J. Biol. Chem. 256: 8987–8993.
Reinhertz, E.L., Kung, P.C., Pesando, J.M., Ritz, J., Goldstein, G., and Schlossman, S.F. 1979. Ia determinants on human T-cell subsets defined by monoclonal antibody.J. Exp. Med. 150: 1472–1482.
Shackeford, D.A., and Strominger J.L. 1980. Demonstration of structural polymorphism among HLA-DR light chains by two-dimensional gel electrophoresis.J. Exp. Med. 150: 1472–1482.
Shackelford, D.A., Lampson, L.A., and Strominger, J.L. 1981. Analysis of HLA-DR antigens by using monoclonal antibodies: recognition of conformational differences in biosynthetic intermediates.J. Immunol. 127: 1403–1410.
Shaw, S., Kavathas, P., Pollack, M.S., Charmot, D., and Mawas, C. 1981. Family studies define a new histocompatibility locus, SB, between HLA-DR and GLO.Nature 293: 745–747.
Suzuki, M., Maeda, H., Mukai, R., Yabe, T., and Hamaguchi, H. 1983. Identification of the MT3 molecule using two-dimensional gel electrophoresis and alloantisera.Immunogenetics 18: 575–583.
Tanigaki, N., Tosi, R., Duquesnoy, R.J., and Ferrara, G.B. 1983. Three Ia species with differen structures and alloantigenic determinants in a HLA-homozygous cell line.J. Exp. Med. 157: 231–247.
Terasaki, P.I., Park, M.S., Bernoco, D., Opelz, G., and Mickey, M.R. 1981. Overview of the 1980 International Histocompatibility Workshop. InHistocompatibility Testing 1980, Terasaki, P.J., ed., UCLA Press, Los Angeles, pp. 1–17.
Yabe, T., Suzuki, M., Satake, M., Juji, T., and Hamaguchi, H. The MT3 specificity is located on a novel human class II antigen distinct from the HLA-DR and DC-like antigens. (submitted)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yabe, T., Suzuki, M., Mukai, R. et al. Analysis of the HLA-DRw9 antigen using two-dimensional gel electrophoresis and alloantisera. Jap J Human Genet 29, 17–25 (1984). https://doi.org/10.1007/BF01876753
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01876753