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Maintenance on a ketogenic diet: voluntary exercise, adiposity and neuroendocrine effects

International Journal of Obesity volume 33, pages 824–830 (2009)Cite this article

Abstract

Background:

Adherence to low-carbohydrate, ketogenic diets (KDs) has been associated with greater weight loss in the short-term than low-fat, calorie-restricted diets. However, consumption of KDs may result in decreased voluntary exercise and thus render long-term weight loss and maintenance of weight loss difficult.

Methods:

Rats were maintained on either a non-ketogenic chow (CH) diet or a low-carbohydrate, KD for 6 weeks. Half of each dietary group was sedentary, whereas the other half was given access to a running wheel. Running wheel activity (total distance and meters per minute), plasma leptin and insulin, adiposity, and hypothalamic mRNA for neuropeptide Y and proopiomelanocortin (POMC) were measured to assess activity-related effects in animals maintained on KD.

Results:

With access to a running wheel, rats on KD engaged in similar levels of voluntary activity as CH rats and both dietary groups decreased caloric intake. Caloric intake increased over time such that it was significantly greater than sedentary controls after 1 month of access to the wheels, however body weight remained decreased. Sedentary rats maintained on KD had increased adiposity and plasma leptin levels and decreased hypothalamic POMC mRNA, as compared to sedentary CH rats. KD rats with access to a running wheel had similar levels of adiposity and plasma leptin levels as CH rats with access to running wheels, but significantly increased POMC mRNA in the arcuate.

Conclusion:

We demonstrate that maintenance on KD does not inhibit voluntary activity in a running wheel. Furthermore, prevention of KD-related increased adiposity and plasma leptin, as measured in sedentary KD rats, significantly increases levels of the anorexigenic neuropeptide POMC mRNA.

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References

  1. Flegal KM, Carroll MD, Ogden CL, Johnson CL . Prevalence and trends in obesity among US adults, 1999–2000. JAMA 2002; 288: 1723–1727.

    Article  Google Scholar 

  2. Serdula MK, Mokdad AH, Williamson DF, Galuska DA, Mendlein JM, Heath GW . Prevalence of attempting weight loss and strategies for controlling weight. JAMA 1999; 282: 1353–1358.

    Article  CAS  Google Scholar 

  3. Nordmann AJ, Nordmann A, Briel M, Keller U, Yancy Jr WS, Brehm BJ et al. Effects of low-carbohydrate vs low-fat diets on weight loss and cardiovascular risk factors: a meta-analysis of randomized controlled trials. Arch Intern Med 2006; 166: 285–293.

    Article  CAS  Google Scholar 

  4. Atkins RC, Ornish D, Wadden T . Low-carb, low-fat diet gurus face off. Interview by Joan Stephenson. JAMA 2003; 289: 1767–1768, 1773.

    Article  Google Scholar 

  5. Atkins RC . Dr. Atkins’ New Diet Revolution. Avon Books: New York, 1992.

    Google Scholar 

  6. Larosa JC, Fry AG, Muesing R, Rosing DR . Effects of high-protein, low-carbohydrate dieting on plasma lipoproteins and body weight. J Am Diet Assoc 1980; 77: 264–270.

    CAS  PubMed  Google Scholar 

  7. Westman EC, Yancy WS, Edman JS, Tomlin KF, Perkins CE . Effect of 6-month adherence to a very low carbohydrate diet program. Am J Med 2002; 113: 30–36.

    Article  Google Scholar 

  8. Brehm BJ, Seeley RJ, Daniels SR, D’Alessio DA . A randomized trial comparing a very low carbohydrate diet and a calorie-restricted low fat diet on body weight and cardiovascular risk factors in healthy women. J Clin Endocrinol Metab 2003; 88: 1617–1623.

    Article  CAS  Google Scholar 

  9. Yancy Jr WS, Olsen MK, Guyton JR, Bakst RP, Westman EC . A low-carbohydrate, ketogenic diet versus a low-fat diet to treat obesity and hyperlipidemia: a randomized, controlled trial. Ann Intern Med 2004; 140: 769–777.

    Article  Google Scholar 

  10. McAuley KA, Hopkins CM, Smith KJ, McLay RT, Williams SM, Taylor RW et al. Comparison of high-fat and high-protein diets with a high-carbohydrate diet in insulin-resistant obese women. Diabetologia 2005; 48: 8–16.

    Article  CAS  Google Scholar 

  11. Foster GD, Wyatt HR, Hill JO, McGuckin BG, Brill C, Mohammed BS et al. A randomized trial of a low-carbohydrate diet for obesity. N Engl J Med 2003; 348: 2082–2090.

    Article  CAS  Google Scholar 

  12. Dansinger ML, Gleason JA, Griffith JL, Selker HP, Schaefer EJ . Comparison of the Atkins, Ornish, Weight Watchers, and Zone diets for weight loss and heart disease risk reduction: a randomized trial. JAMA 2005; 293: 43–53.

    Article  CAS  Google Scholar 

  13. Klem ML, Wing RR, McGuire MT, Seagle HM, Hill JO . A descriptive study of individuals successful at long-term maintenance of substantial weight loss. Am J Clin Nutr 1997; 66: 239–246.

    Article  CAS  Google Scholar 

  14. Wing RR . Physical activity in the treatment of the adulthood overweight and obesity: current evidence and research issues. Med Sci Sports Exerc 1999; 31: S547–S552.

    Article  CAS  Google Scholar 

  15. Keith RE, O’Keeffe KA, Blessing DL, Wilson GD . Alterations in dietary carbohydrate, protein, and fat intake and mood state in trained female cyclists. Med Sci Sports Exerc 1991; 23: 212–216.

    Article  CAS  Google Scholar 

  16. White AM, Johnston CS, Swan PD, Tjonn SL, Sears B . Blood ketones are directly related to fatigue and perceived effort during exercise in overweight adults adhering to low-carbohydrate diets for weight loss: a pilot study. J Am Diet Assoc 2007; 107: 1792–1796.

    Article  CAS  Google Scholar 

  17. George V, Tremblay A, Despres JP, Leblanc C, Bouchard C . Effect of dietary fat content on total and regional adiposity in men and women. Int J Obes 1990; 14: 1085–1094.

    CAS  PubMed  Google Scholar 

  18. Horton TJ, Drougas H, Brachey A, Reed GW, Peters JC, Hill JO . Fat and carbohydrate overfeeding in humans: different effects on energy storage. Am J Clin Nutr 1995; 62: 19–29.

    Article  CAS  Google Scholar 

  19. Lean ME, James WP . Metabolic effects of isoenergetic nutrient exchange over 24 hours in relation to obesity in women. Int J Obes 1988; 12: 15–27.

    CAS  PubMed  Google Scholar 

  20. Lissner L, Heitmann BL . Dietary fat and obesity: evidence from epidemiology. Eur J Clin Nutr 1995; 49: 79–90.

    CAS  Google Scholar 

  21. Macdiarmid JI, Cade JE, Blundell JE . High and low fat consumers, their macronutrient intake and body mass index: further analysis of the National Diet and Nutrition Survey of British Adults. Eur J Clin Nutr 1996; 50: 505–512.

    CAS  PubMed  Google Scholar 

  22. Woods SC, D’Alessio DA, Tso P, Rushing PA, Clegg DJ, Benoit SC et al. Consumption of a high-fat diet alters the homeostatic regulation of energy balance. Physiol Behav 2004; 83: 573–578.

    Article  CAS  Google Scholar 

  23. Kinzig KP, Scott KA, Hyun J, Bi S, Moran TH . Altered hypothalamic signaling and responses to food deprivation in rats fed a low-carbohydrate diet. Obes Res 2005; 13: 1672–1682.

    Article  CAS  Google Scholar 

  24. Levitsky DA . Feeding patterns of rats in response to fasts and changes in environmental conditions. Physiol Behav 1970; 5: 291–300.

    Article  CAS  Google Scholar 

  25. Looy H, Eikelboom R . Wheel running, food intake, and body weight in male rats. Physiol Behav 1989; 45: 403–405.

    Article  CAS  Google Scholar 

  26. Mueller DT, Loft A, Eikelboom R . Alternate-day wheel access: effects on feeding, body weight, and running. Physiol Behav 1997; 62: 905–908.

    Article  CAS  Google Scholar 

  27. Levin BE, Dunn-Meynell AA . Differential effects of exercise on body weight gain and adiposity in obesity-prone and -resistant rats. Int J Obes (London) 2006; 30: 722–727.

    Article  CAS  Google Scholar 

  28. Keith RE, O’Keeffe KA, Alt LA, Young KL . Dietary status of trained female cyclists. J Am Diet Assoc 1989; 89: 1620–1623.

    CAS  PubMed  Google Scholar 

  29. Butki BD, Baumstark J, Driver S . Effects of a carbohydrate-restricted diet on affective responses to acute exercise among physically active participants. Percept Mot Skills 2003; 96: 607–615.

    Article  Google Scholar 

  30. Cheung CC, Clifton DK, Steiner RA . Proopiomelanocortin neurons are direct targets for leptin in the hypothalamus. Endocrinology 1997; 138: 4489–4492.

    Article  CAS  Google Scholar 

  31. Schwartz MW, Seeley RJ, Woods SC, Weigle DS, Campfield LA, Burn P et al. Leptin increases hypothalamic pro-opiomelanocortin mRNA expression in the rostral arcuate nucleus. Diabetes 1997; 46: 2119–2123.

    Article  CAS  Google Scholar 

  32. Hagan MM, Rushing PA, Schwartz MW, Yagaloff KA, Burn P, Woods SC et al. Role of the CNS melanocortin system in the response to overfeeding. J Neurosci 1999; 19: 2362–2367.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This research was supported by NIH grant DK078654 (KPK). We thank Sara Hargrave, Mary Ann Honors and Melissa McCurley for technical assistance.

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  1. Department of Psychological Sciences, Ingestive Behavior Research Center, Purdue University, West Lafayette, IN, USA

    K P Kinzig & R J Taylor

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  1. K P Kinzig
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Correspondence to K P Kinzig.

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Kinzig, K., Taylor, R. Maintenance on a ketogenic diet: voluntary exercise, adiposity and neuroendocrine effects. Int J Obes 33, 824–830 (2009). https://doi.org/10.1038/ijo.2009.109

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