Abstract
The RAPD PCR technique has been employed to investigate phylogenetic relationships between species of the genera Lolium and Festuca. Several decamer primers were used to generate patterns from groups of genotypes of several different species. The degree of band sharing was used to evaluate genetic distances between species and to construct a phylogenetic tree which is in good overall agreement with classical taxonomy, but contains a number of novel insights. The degree of homoplasy inherent in this approach has been investigated using Southern hybridization. These results are discussed in the context of current work in molecular biosystematics.
Similar content being viewed by others
Article PDF
References
Borrill, M. 1972. Studies in Festuca. III. The contribution of F. scariosa to the evolution of the polyploids in sections Bovinae and Scariosae. New Phytol, 71, 523–532.
Borrill, M. 1976. Temperate grasses. In: Simmons, N. W. (ed.) Evolution of Crop Plants, pp. 137–142. Longman, London.
Borrill, M, Kirby, M, and Morgan, G M. 1977. Studies in Festuca. II. Interrelationship of some putative diploid ancestors of the polyploid broad-leaved fescues. New Phytol, 78, 661–674.
Bulinska-Radomska, Z, and Lester, R N. 1986. Intergeneric relationships of Lolium, Festuca and Vulpia (Poaceae) and their phylogeny. Plant Syst Evol, 159, 217–227.
Caetano-Anolles, G, Bassam, B J, and Gresshoff, P M. 1991. DNA amplification fingerprinting using very short arbitrary oligonucleotide primers. Bio/Technology, 9, 553–556.
Demeke, T, Adams, R P, Chibbar, and R. 1992. Potential taxonomic use of random amplified polymorphic DNA (RAPD): a case history in Brassica. Theor Appl Genet, 84, 990–994.
Dowling, T E, Moritz, C, and Palmer, J D. 1990. Nucleic acids: restriction site analysis. In: Hillis, D. M. and Moritz, C. (eds) Molecular Systematics, pp. 250–318. Sinauer Associates, Sunderland, MA.
Evans, G M, Hayward, M D, Forster, J W, McAdam, N J, Scanlon, M J, Stammers, M, and Will, J A K. 1991. Genome analysis and its manipulation in Lolium. In: De Nijs, A. P. M. and Elgersma, A. (eds) Fodder Crops Breeding: Achievements, Novel Strategies and Biotechnology, Proceedings of the 16th meeting of the fodder crops section of Eucarpia, Wageningen, Netherlands, 18–22 November 1990, pp. 141–146. Pudoc, Wageningen.
Feinberg, A P, and Vogelstein, A P. 1984. A technique for radiolabelling DNA restriction fragments to high specific activity (Addendum). Analyt Biochem, 137, 266–267.
Felsenstein, J. 1990. PHYLIP manual version 3.3. University Herbarium, University of California, Berkeley, CA.
Fitch, W M, and Margoliash, E. 1967. Construction of phylogenetic trees. Science, 155, 279–284.
Jauhar, P P. 1976. Chromosome pairing in some triploid and trispecific hybrids in Lolium I Festuca and its phylogenetic implications. Chromosomes Today, 5, 165–177.
Lehvaslaiho, H, Saura, A, and Lokki, J. 1987. Chloroplast DNA variation in the grass tribe Festucae. Theor Appl Genet, 74, 298–302.
Malik, C P, and Thomas, P T. 1967. Cytological relationships and genome structure of some Festuca species. Caryologia, 209, 1–39.
Miller, J C, and Tanksley, S D. 1990. RFLP analysis of phylogenetic relationships and genetic variation in the genus Lycopersicon. Theor Appl Genet, 80, 437–448.
Murray, M G, and Thompson, W F. 1980. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 8, 4321.
Naylor, B. 1960. Species differentiation in genus Lolium. Heredity, 15, 219–223.
Nei, M. 1987. Molecular Evolutionary Genetics. Columbia University Press, New York.
Reed, K C. 1986. Alkaline Southern blotting to Zetaprobe membrane. Bio-Rad Bulletin, no, 1233.
Saghai-Maroof, M A, Soliman, K M, Jorgensen, R A, and Allard, R W. 1984. Ribosomal DNA spacer length polymorphisms in barley: Mendelian inheritance, chromosome location and population dynamics. Proc Natl Acad Sci USA, 81, 8014–8018.
Sandery, M J, Forster, J W, Blunden, R, and Jones, R N. 1990. Identification of a family of repeated sequences on the rye B-chromosome. Genome, 33, 908–913.
Seal, A G. 1981. Cytogenetic Studies in Festuca end Lolium. Ph.D. Thesis, University of Wales.
Stammers, M. 1992. Molecular Mapping and Biosystematics of the Festuceae. Ph.D. Thesis, University of Wales.
Terrel, E E. 1968. A taxonomic revision of the genus Lolium. USARS Techn Bull, 1392, 1–65.
Thomas, H, and Humphreys, M O. 1991. Progress and potential of interspecific hybrids of Lolium and Festuca. J Agric Sci Camb, 117, 1–8.
Williams, J G K, Kubelik, A R, Livak, K J, Rafalski, J A, and Tingey, S V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl Acids Res, 18, 6531–6535.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Stammers, M., Harris, J., Evans, G. et al. Use of random PCR (RAPD) technology to analyse phylogenetic relationships in the Lolium/Festuca complex. Heredity 74, 19–27 (1995). https://doi.org/10.1038/hdy.1995.3
Received:
Issue Date:
DOI: https://doi.org/10.1038/hdy.1995.3
Keywords
This article is cited by
-
Mapping QTL for summer dormancy related traits in tall fescue (Festuca arundinacea Schreb.)
Scientific Reports (2020)
-
Interspecific molecular variation of Tunisian complex Lolium perenne L. and Festuca arundinacea Schreb. based on the internal transcribed spacer locus (ITS)
Physiology and Molecular Biology of Plants (2020)
-
Phylogenetic analysis of Festuca–Lolium complex using SRAP markers
Genetic Resources and Crop Evolution (2016)
-
Comparative transcriptome analysis within the Lolium/Festuca species complex reveals high sequence conservation
BMC Genomics (2015)
-
Leaf epidermal characteristics and genetic variability in Central European populations of broad-leaved Festuca L. taxa
Plant Systematics and Evolution (2014)