Abstract
Surviving M1 plants derived from pollination with irradiated pollen were selfed to produce the second (M2) generation. In three quantitative traits, the means and phenotypic frequency distributions of the M2 populations were identical to that of the F2 population. In five major gene traits, all but six of the 244 M2 segregations were in the expected ratios is the F2's. Aberrant segregations produced both excess paternal as well as maternal phenotypes. There was no consistent trend of a “maternal shift” in the M2 generation that may render the technique of irradiated pollination useful by preferentially producing pure breeding maternal materials having a few specific paternal characteristics. Overall, pollination with irradiated pollen in rice produced mainly normal hybrid progeny with very little heritable variations. These lack of responses in rice may be explained by the fact that rice pollen is relatively insensitive to irradiation on the one hand, and that the pollen genome is less able to accommodate mutational damage on the other hand. Thus, the rice plant is considered less amenable to the application of irradiated pollination as a practical breeding technique.
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Chin, SF., Gordon, G. Pollination with irradiated pollen in rice—Oryza sativa L. II. The second (M2) generation. Heredity 63, 171–179 (1989). https://doi.org/10.1038/hdy.1989.89
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DOI: https://doi.org/10.1038/hdy.1989.89