Abstract
A non-radioactive in situ hybridization method using biotin-labelled rDNA has made it possible to localize rRNA genes not only at the secondary constriction in both homologous chromosomes No. 3 of Crepis capillaris but also in the B chromosomes occurring in the plants employed. Very clear dot-like rDNA signals at the telomeres of both arms were observed in all B chromosomes. Histochemical silver staining, which is indicative of transcriptional activity of rRNA gene clusters, resulted in both darkly-staining nucleolar constrictions of chromosomes No. 3 and silver deposits at the telomeres of Bs. We conclude that the B chromosomes of C. capillaris are isochromosomes with active rRNA genes located near both telomeres.
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Maluszynska, J., Schweizer, D. Ribosomal RNA genes in B chromosomes of Crepis capillaris detected by non-radioactive in situ hybridization. Heredity 62, 59–65 (1989). https://doi.org/10.1038/hdy.1989.8
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DOI: https://doi.org/10.1038/hdy.1989.8
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