Summary
Single copy DNA sequence homologies were measured by interspecific molecular hybridization and thermal denaturation techniques for eight species of the genus Atriplex and spinach (Spinacia oleracea). Thermal stability profiles for Atriplex interspecific hybrids indicated more base pair mismatch than has been observed in most previously reported intrageneric comparisons of animal DNA. On the assumption that sequence divergence by base substitution is proportional to the time which has elapsed subsequent to speciation, the data are interpreted as indicating that lines leading to many modern Atriplex species probably originated during a single period of rapid speciation. This group includes C3 and C4 photosynthetic types from both subgenera. Thus the molecular data do not support the classical subgeneric distinction, and it is no longer necessary to postulate a polyphyletic origin for C4 photosynthesis within Atriplex.
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This research was supported in part by NSF grant DEB76-83405 and funds from the Carnegie Institution of Washington. This paper is Publication No. 717 from the Department of Plant Biology, and is based on a portion of a dissertation submitted by H.S.B. in partial fulfilment of the Ph.D requirements in the Department of Botany, University of Massachusetts, Amherst.
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Belford, H., Thompson, W. Single copy DNA homologies in Atriplex. II. Hybrid thermal stabilities and molecular phylogeny. Heredity 46, 109–122 (1981). https://doi.org/10.1038/hdy.1981.10
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DOI: https://doi.org/10.1038/hdy.1981.10
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