Summary
Haploid cultures of Dictyostelium discoideum obtained from the axenic strain Ax-2 were fused with each other and also with the non-axenic strain X9 to produce diploid clones. The diploids were heterozygous for two recessive drug resistance genes and thus by measuring the segregation under non-selective conditions of haploids expressing drug resistance, a measure of the frequency of haploidisation was obtained. It was demonstrated that X9 when fused with an axenic strain produced diploids of greater stability than those obtained by the fusion of two axenic strains. It has been postulated that X9 carries a gene (or a series of genes) that give rise to diploid stability
Article PDF
References
Bonner, J T. 1959. The Cellular Slime Moulds. Princeton University Press.
Brody, T, and Williams, K L. 1974. Cytological analysis of the parasexual cycle in Dictyostelium discoideum. J Gen Microbiol 82, 371–383.
Garrod, D, and Ashworth, J M. 1973. Development of the cellular slime mould Dictyostelium discoideum. In Microbial Differentiation. Symp Soc Gen Microbiol, 23, 407–435.
Gingold, E A, and Ashworth, J M. 1974. Evidence for Mitotic Crossing-over during the parasexual cycle of the cellular slime mould Dictyostelium discoideum. J Gen Microbiol. 84, 70–78.
Katz, E R, and Sussman, M. 1972. Parasexual recombination in Dictyostelium discoideum; selection of stable diploid heterozygotes and stable segregants. Proc Natl Acad Sci US, 69, 495–497.
Loomis, W F. 1969. Temperature-sensitive mutants of Dictyostelium discoideum. J Bacteriol, 99, 65–69.
Raper, K. 1935. Dictyostelium discoideum, a new species of slime mould from decaying forest leaves. J Agric Res, 50. 135–147.
Ross, I K. 1960. Studies on diploid strains of Dictyostelium discoideum. Amer J Bot, 47, 54–59.
Sinha, U, and Ashworth, J M. 1969. Evidence for the existence of elements of a para-sexual cycle in the cellular slime mould Dictyostelium discoideum. Proc Roy Soc, Series B, 173, 531–540.
Sussman, M, and Sussman, R R. 1962. Ploidal inheritance in Dictyostelium discoideum: Stable haploid, stable diploid and metastable strains. J Gen Microbiol, 28, 417–429.
Watts, D J, and Ashworth, J M. 1970. Growth of myxamoebae of the cellular slime mould Dictyostelium discoideum in axenic culture. Biochem J, 119, 171–174.
Williams, K L, Kessin, R H, and Newell, P C. 1974. Parasexual genetics in Dictyostelium discoideum: Assignment to linkage groups of genes for acriflavin resistance and growth in axenic medium. J Gen Microbiol 84, 59–69.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gingold, E. Stability of diploid clones of the cellular slime mould Dictyostelium discoideum. Heredity 33, 419–423 (1974). https://doi.org/10.1038/hdy.1974.110
Received:
Issue Date:
DOI: https://doi.org/10.1038/hdy.1974.110
This article is cited by
-
Linkage analysis of developmental mutations in aggregation-deficient mutants of Dictyostelium discoideum
MGG Molecular & General Genetics (1977)