Abstract
Purpose To study alterations in conjunctival collagen in the conjunctiva of patients with active trachoma.
Methods We studied conjunctival biopsy specimens obtained from nine subjects with active trachoma and from four control subjects. We used immunohistochemical techniques and a panel of monoclonal and polyclonal antibodies directed against types I, III, IV and V collagen.
Results In normal conjunctiva, the staining for types I and III collagen was localised to the substantia propria. Type IV collagen was located in the epithelial and capillary endothelial basement membranes. The staining for type V collagen was absent. In trachoma biopsy specimens, staining for types I and III collagen showed collagen fibrils among epithelial cells, patchy increase in staining intensity in the upper stroma, and thicker and irregularly arranged collagen fibrils in the substantia propria. Staining for type IV collagen showed irregularly thickened epithelial basement membrane. Staining for type V collagen showed patchy staining in the upper substantia propria; it was also noted in the cytoplasm of fibroblasts, in the walls of blood vessels in the substantia propria, and in the walls of accessory lacrimal glands.
Conclusions Our data indicate new type V collagen formation, and increased types I, III and IV collagen content, in the conjunctiva from patients with active trachoma.
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References
Dawson CR, Jones BR, Tarizzo ML . Guide to trachoma control in programmes for the prevention of blindness. Geneva: World Health Organization; Albany, NY: WHO Publications Centre USA [distributor], 1981.
Taylor HR . Trachoma and inclusion conjunctivitis. In: Strickland G, editor. Hunter's tropical medicine. 7th ed. Philadelphia: WB Saunders, 1991:288–93.
Abu El-Asrar AM, Van den Oord JJ, Geboes K, Missotten L, Emarah MH, Desmet V . Immunopathology of trachomatous conjunctivitis. Br J Ophthalmol 1989;73:276–82.
Abu El-Asrar AM, Emarah MH, Van den Oord JJ, Geboes K, Desmet V, Missotten L . Conjunctival epithelial cells infected with Chlamydia trachomatis express HLA-DR antigens [letter]. Br J Ophthalmol 1989;73:399–400.
Reacher MH, Pe'er J, Rapoza PA, Whittum-Hudson JA, Taylor HR . T cells and trachoma: their role in cicatricial disease. Ophthalmology 1991;98:334–41.
Freundlich B, Bomalski JS, Neilson E, Jimenez SA . Regulation of fibroblast proliferation and collagen synthesis by cytokines. Immunol Today 1986;7:303–7.
Werkmeister JA, Ramshaw AM . Monoclonal antibodies to type V collagen for immunohistological examination of new tissue deposition associated with biomaterial implants. J Histochem Cytochem 1991;39:1215–20.
Honda M, Goto Y, Kuzuo H, Ishikawa S, Morioka S, Yamori Y, et al. Biochemical remodelling of collagen in the heart of spontaneously hypertensive rats: prominent increase in type V collagen. Jpn Circ J 1993;57:434–41.
Konomi H, Arima M, Tanaka H, Hayashi T, Ikeda S . Increased deposition of types III and V collagen in neurofibroma tissue from patients with von Recklinghausen disease. Brain Dev 1989;11:378–83.
Asokan R, Reddy GK, Dhar SC . Neoplastic association of enhanced type V collagen production in rat fibrosarcoma. Mol Cell Biochem 1993;120:25–32.
Graham M, Diegelmann R, Elson C, Lindblad W, Gostschalk N, Gay S, et al. Collagen content and types in the intestinal strictures of Crohn's disease. Gastroenterology 1988;94:257–65.
Murata K, Motayama T, Kotake C . Collagen types in various layers of the human aorta and their changes with the atherosclerotic process. Atherosclerosis 1986;60:251–62.
Shekhonin BV, Domogatsky SP, Muzykantov VR, Idelson GL, Rukosuev VS . Distribution of type I, III, IV and V collagen in normal and atherosclerotic human arterial wall: immunomorphological characteristics. Collagen Rel Res 1985;5:355–68.
Leonardi A, Abatangelo G, Cortivo R, Secchi AG . Collagen types I and III in giant papillae of vernal keratoconjunctivitis. Br J Ophthalmol 1995;79:482–5.
Cintron C, Hong B-S, Covington HI, Macarak EJ . Heterogeneity of collagens in rabbit cornea: type III collagen. Invest Ophthalmol Vis Sci 1988;29:767–75.
Narayanan AS, Engel LD, Page RC . The effect of chronic inflammation on the composition of collagen types in human connective tissue. Collagen Rel Res 1983;3:323–34.
Dutt JE, Ledoux D, Baer H, Foster CS . Collagen abnormalities in conjunctiva of patients with cicatricial pemphigoid. Cornea 1996;15:606–11.
Burgeson R, Adil FA, Kaitila II, Hollister DW . Fetal membrane collagens: identification of two new collagen alpha chains. Proc Natl Acad Sci USA 1976;73:2579–83.
Chung E, Rhodes RK, Miller EJ . Isolation of three collagenous components of probable basement membrane origin from several tissues. Biochem Biophys Res Commun 1976;71:1167–74.
Birk DE, Fitch JM, Linsenmayer TF . Organization of collagen types I and V in the embryonic chicken cornea. Invest Ophthalmol Vis Sci 1986;27:1470–7.
Gordon MK, Foley JW, Birk DE, Fitch JM, Linsenmayer TF . Type V collagen and Bowman's membrane: quantitation of mRNA in corneal epithelium and stroma. J Biol Chem 1994;269:24959–66.
Martinez-Hernandez A, Gay S, Miller EJ . Ultrastructural localization of type V collagen in rat kidney. J Cell Biol 1982;92:343–9.
Ruggiero F, Champliaud MF, Garrone R, Aumailley M . Interactions between cells and collagen V molecules or single chains involve distinct mechanisms. Exp Cell Res 1994;210:215–23.
Le Barou RG, Hook A, Esko JD, Gay S, Hook M . Binding of heparan sulphate to type V collagen: a mechanism of cell-substrate adhesion. J Biol Chem 1989;264:7950–6.
Birk DE, Fitch JM, Babiarz JP, Doane KJ, Linsenmayer TF . Collagen fibrillogenesis in vitro: interaction of types I and V collagen regulates fibril diameter. J Cell Sci 1990;95:649–57.
Andrikopoulos K, Liu X, Keene DR, Jaenisch R, Ramirez F . Targeted mutation in the CO15a2 gene reveals a regulatory role for type V collagen during matrix assembly. Nature Genet 1995;9:31–6.
McLaughlin JS, Linsenmayer TF, Birk DE . Type V collagen synthesis and deposition by chicken embryo corneal fibroblasts in vitro. J Cell Sci 1989;94:371–9.
Lawrence R, Hartmann DJ, Sonenshein GE . Transforming growth factor (31 stimulates type V collagen expression in bovine vascular smooth muscle cells. J Biol Chem 1994;269:9603–9.
Okada Y, Katsuda S, Watanabe H, Nakanishi I . Collagen synthesis of human arterial smooth muscle cells: effects of platelet-derived growth factor, transforming growth factor-beta 1 and interleukin-1. Acta Pathol Jpn 1993;43:160–7.
Silver MH, Murray JC, Pratt RM . Epidermal growth factor stimulates type-V collagen synthesis in cultured murine palatal shelves. Differentiation (Camb.) 1984;27:205–8.
Choe I, Aycock RS, Raghow R, Myers JC, Seyer JM, Kang AH . A hepatic fibrogenic factor stimulates the synthesis of types I, III and V procollagens in cultured cells. J Biol Chem 1987;262:5408–13.
Marshall GE, Konstas AGP, Lee WR . Collagens in ocular tissues. Br J Ophthalmol 1993;77:515–24.
Dake Y, Mukae R, Soda Y, Kaneko M, Amemiya T . Immunohistochemical localization of collagen types I, II, III and IV in pterygium tissues. Acta Histochem 1989;87:71–4.
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El-Asrar, A., Geboes, K., Al-Kharashi, S. et al. Collagen content and types in trachomatous conjunctivitis. Eye 12, 735–739 (1998). https://doi.org/10.1038/eye.1998.179
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DOI: https://doi.org/10.1038/eye.1998.179