Abstract
Sections of human anterior optic nerve and nerve head were incubated in a physiological solution containing a radiolabelled beta blocker at a low concentration. The beta blocker used was (-)-(125iodo)-cyanopindolol, which has a high affinity and specificity for beta-adrenergic receptors. Concurrent incubations were performed with a great excess of unlabelled beta blocker added to demonstrate non-specific binding. Following incubation the sections were washed and dried. They were then apposed to photographic film for 5 days and developed. Incubations were performed with the stereoisomers of propranolol and an alpha blocker as well as specific beta-one and beta-two blockers. Beta-adrenergic receptors were demonstrated in anterior optic nerve and optic nerve head. The majority were of the beta-two subtype.
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Dawidek, G., Robinson, M. Beta-adrenergic receptors in human anterior optic nerve: An autoradiographic study. Eye 7, 122–126 (1993). https://doi.org/10.1038/eye.1993.25
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DOI: https://doi.org/10.1038/eye.1993.25