Abstract
A study was performed on the commensal external eye flora in 34 long-term contact lens wearers and a matched control group, in order to identify the effect of lens wear. Samples were taken from the lid margin and conjunctiva by semi-quantitative, and tear film by quantitative methods. Cultured bacteria were identified with particular attention to coagulase-negative staphylococci. No qualitative alteration in the commensal bacteria was found, although lens wearers were found to have a significantly higher number of species at all sites than controls. Significant quantitative changes were identified on the lid margin, with particularly high counts in some lens wearers, and tear film. Increased numbers of bacteria obtained from the conjunctiva were not statistically significant. Quantitative changes in the tear film are thought to be secondary to changes at the lid margin, for which no explanation is apparent.
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Locatcher-Khorazo D and Seegal BC : Microbiology of the eye. St Louis. CV Mosby, 1972: 13–23.
Allansmith MR, Ostler B, Butterworth M : Concomitance of bacteria in various areas of the eye. Arch Ophthalmol 1969, 82: 37–42.
Noble WC : Microbiology of the human skin. 2nd ed. London. Lloyd-Luke, 1981.
Rauschl RT and Rogers JJ : The effect of hydrophilic contact lens wear on the bacterial flora of the human conjunctiva. Int Contact Lens Clin 1978, 5: 56–62.
McBride ME : Evaluation of the microbial flora of the eye during wear of soft contact lenses. Appl Environ Microbiol 1979, 37: 233–6.
Smolin G, Okumoto M, Nozik RA : The microbial flora in extended-wear soft contact lens wearers. Am J Ophthalmol 1979, 88: 543–7.
Callender MG, Tse LSY, Charles AM, Lutz D : Bacterial flora of the eye and contact lens cases during hydrogel lens wear. Am J Optom Physiol Optics 1986, 63: 177–80.
Hovding G : The conjunctival and contact lens bacterial flora during lens wear. Acta Ophthalmol 1981, 59: 387–401.
Morgan JF : Complications associated with contact lens solutions. Ophthalmology 1979, 86: 1107–19.
Centers for Disease Control: Acanthamoeba: keratitis associated with contact lenses—United States. MMWR 1986, 35: 405–8.
Moore MB, McCulley JP, Newton C, et al: Acanthamoeba: keratitis: A growing problem in soft and hard contact lens wearers. Ophthalmology 1987, 94: 1654–61.
Cagle GD and Abshire RL : Quantitative ocular bacteriology: a method for the enumeration and identification of bacteria from the skinlash margin and conjunctiva. Invest Ophthalmol Vis Sci 1981, 20: 751–7.
Schleifer KH (Ed): Section 12. Grampositive cocci. In: Sneath PHA, Mair NS, Sharpe ME, Holt JG eds. Bergey's Manual of Systematic Bacteriology:, Vol 2, Baltimore: Williams and Wilkins 1986: 999–1103.
Mayo MS, Schlitzer RL, Ward MA, Wilson LA, Ahearn DG : Association of Pseudomonas: and Serratia: corneal ulcers with use of contaminated solutions. J Clin Microbiol 1987, 25: 1398–400.
Donzis PB, Mondino BJ, Weissman BA, Bruckner DA : Microbial contamination of contact lens care systems. Am J Ophthalmol 1987, 104: 325–33.
Larkin DFP, Kilvington S, Easty DL : Contamination of contact lens storage cases by Acanthamoeba: and bacteria. Br J Ophthalmol 1990, 74: 133–5.
Mayo MS, Cook WL, Schlitzer RL, Ward MA, Wilson LA, Ahearn DG : Antibiograms, serotypes and plasmid profiles of Pseudomonas aeruginosa: associated with corneal ulcers and contact lens wear. Clin Microbiol 1986, 24: 372–6.
Larkin DFP and Easty DL . External eye bacteria as a nutrient source for Acanthamoeba. Graefe's Arch Clin Exp Ophthalmol 1990, 228: [in press].
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Larkin, D., Leeming, J. Quantitative alterations of the commensal eye bacteria in contact lens wear. Eye 5, 70–74 (1991). https://doi.org/10.1038/eye.1991.14
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DOI: https://doi.org/10.1038/eye.1991.14
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