Abstract
To determine the cellular composition of subretinal membranes in proliferative vitreoretinopathy, surgically excised membranes were studied using electron microscopic and immunohistochemical procedures. Light microscopic immunohistochemistry was found to be superior to conventional electron microscopy as a means of evaluating the relative contributions of retinal pigment epithelium and glia to subretinal membranes. The cells of subretinal membranes chiefly comprised retinal pigment epithelium while glial cells were either absent or contributed only a minor component. It is proposed that the clinical properties of subretinal membranes in proliferative vitreoretinopathy derive from their retinal pigment epithelial cell element.
Similar content being viewed by others
Article PDF
References
Winthrop SR, Cleary PE, Minckler DS, Ryan SJ : Penetrating eye injuries: A histopathological review. Br J Ophthalmol 1980, 64: 809–17.
Trese MT, Chandler D, Machemer R, Charles S : Symptomatic sub-retinal strands ultrastructure and cell behaviour. AVRO abstracts 1983, 24: 291.
Federman JL, Foldberg R, Ridley M, Arbizo VA : Subretinal cellular bands. Trans Am Ophthalmol Soc 1983, 81: 172–9.
Daicker B : Constricting retroretinal membranes associated with traumatic retinal detachments. Graefe's Arch Clin Exp Ophthalmol 1985, 222: 147–53.
Trese MT, Chandler DB, Machemer R : Subretinal strands: Ultrastructural features. Graefe's Arch Clin Exp Ophthalmol 1985, 223: 35–40.
Morino I, Kazusa R, Yamanaka A : Glial cells in subretinal strand. J New Ophthalmol (Atarashii Ganka) 1987, 4: 279–82.
Wilkes SR, Mansour AM, Green WR : Proliferative vitreoretinopathy histopathology of retroretinal membranes. Retina 1987, 7: 94–101.
Bornfield N, El-Hifnawi E, Laqua H : Ultrastructure of subretinal membranes in PVR. International Symposium on PVR 1988, 4.8.
Kampik A . Kenyon KR, Michels RG, Green WR, de la Cruz ZC : Epiretinal and vitreous membranes comparative study of 56. cases. Arch Ophthalmol 1981, 99: 1445–54.
Hiscott PS, Grierson I, McLeod D : Retinal pigment epithelial cells in epiretinal membranes: An immunohistochemical study. Br J Ophthalmol 1984, 68: 708–15.
Hiscott PS, Grierson I, Trombetta CJ, Rahi AHS, Marshall J, McLeod D : Retinal and epiretinal glia—an immunohistochemical study. Br J Ophthalmol 1984, 68: 698–707.
McKechnie NM, Boulton M, Robey HL, Savage FJ, Grierson I : The cytoskeletal elements of human retinal pigment epithelium: In Vitro and In Vivo. J Cell Sci 1988, 91: 303–12.
Owaribe K . Kartenbeck J, Rungger-Brandle E, Franke WW : Cytoskeletons of retinal pigment epithelial cells: interspecies differences of expression patterns indicate independence of cell function from the specific complement of cytoskeletal proteins. Cell Tissue Res 1988, 254: 301–15.
Giorno R : A comparison of two immunoperoxidase staining methods based on the avidin-biotin interaction. Diag Immunol 1984, 2: 161–6.
Shirakawa H, Yoshimura N, Yakakawa R, Matsumura M, Okada M, Ogino N, Ogina N : Cell components in proliferative vitreoretinopathy: Immunofluorescent double staining of cultured cells from proliferative tissues. Ophthalmologica 1987, 194: 56–62.
Matsumura M, Yamakawa R, Yoshimura N, Shirakawa H, Okada M, Ogino N : Subretinal Strands Tissue culture and histological study. Graefe's Arch Clin Exp Ophthalmol 1987, 225: 341–5.
Hiscott PS, Grierson I, Hitchins CA, Rahi AHS, McLeod D : Epiretinal membranes in vitro. Trans Ophthalmol Soc UK 1983, 103: 89–102.
Sternberg P and Machemer R : Subretinal proliferation. Am J Ophthalmol 1984, 98: 456–62.
Machemer R : Discussion of presentation by Federman JL, Foldberg R, Ridley M, Arbizo VA. Subretinal cellular bands. Trans Ophthalmol Soc UK 1983, 81: 172–80.
Foos RY : Vitreoretinal juncture—simple epiretinal membranes. Graefe's Arch Clin Exp Ophthalmol 1974, 189: 231–50.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hiscott, P., Morino, I., Alexander, R. et al. Cellular components of subretinal membranes in proliferative vitreoretinopathy. Eye 3, 606–610 (1989). https://doi.org/10.1038/eye.1989.94
Issue Date:
DOI: https://doi.org/10.1038/eye.1989.94
This article is cited by
-
P38 inhibition reverses TGFβ1 and TNFα-induced contraction in a model of proliferative vitreoretinopathy
Communications Biology (2019)
-
Stage specificity of novel growth factor expression during development of proliferative vitreoretinopathy
Eye (2007)
-
Pathobiology of epiretinal and subretinal membranes: possible roles for the matricellular proteins thrombospondin 1 and osteonectin (SPARC)
Eye (2002)
-
Challenges in ophthalmic pathology: The vitreoretinal membrane biopsy
Eye (2000)
-
Non-vascular vitreoretinopathy: The cells and the cellular basis of contraction
Eye (1996)