Abstract
Hemin blocked lipid peroxidations induced by either ascorbate/FeSO4, a metal-catalyzed oxidation system, or 2,2'-azobis-2-amidino-propane hydrochloride (ABAP) which produces peroxy radicals at constant rates. Hemin at very low micromolar concentrations strongly inhibited the ascorbate/FeSO4-induced peroxidation of rat liver phopholipids, soybean phosphatidylcholine and arachidonic acid, and this inhibition was also evident with the use of ABAP, although much higher concentrations of hemin were required than those for the inhibition of ascorbate/FeSO4-induced lipid peroxidation. However, hemoproteins such as hemoglobin, myoglobin and cytochrome C did not show any significant effect on this lipid peroxidation. Hemopexin and albumin abolished the inhibitory action of hemin. During incubation with ascorbate/FeSO4 or ABAP, hemin underwent a change in its absorption spectrum, resulting in a progressive decrease in the peak height of the characteristic absorption band at 385 nm. The above results suggest that hemin may act as an important antioxidant in vivo, protecting lipids from the peroxidative damage.
Similar content being viewed by others
Article PDF
Author information
Authors and Affiliations
Rights and permissions
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
About this article
Cite this article
Jung, YD., Chay, KO., Song, DU. et al. Hemin inhibits lipid peroxidation induced by ascorbate/FeSO4 and 2,2'-azobis-2-amidino-propane hydrochloride (ABAP). Exp Mol Med 29, 171–175 (1997). https://doi.org/10.1038/emm.1997.26
Published:
Issue Date:
DOI: https://doi.org/10.1038/emm.1997.26
Keywords
This article is cited by
-
Involvement of heme oxygenase-1 in ?-cyclodextrin?hemin complex-induced cucumber adventitious rooting process
Plant Cell Reports (2012)
-
Presoaking with hemin improves salinity tolerance during wheat seed germination
Acta Physiologiae Plantarum (2011)
-
Induction of heme oxygenase-1 with ?-CD-hemin complex mitigates cadmium-induced oxidative damage in the roots of Medicago sativa
Plant and Soil (2011)
-
Apolipoprotein B binds ferritin by hemin-mediated binding: evidence of direct binding of apolipoprotein B and ferritin to hemin
BioMetals (2008)