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Lipids and Cardiovascular/Metabolic Health

Acute differential effects of milk-derived dietary proteins on postprandial lipaemia in obese non-diabetic subjects

European Journal of Clinical Nutrition volume 66, pages 32–38 (2012)Cite this article

Abstract

Background/Objectives:

Postprandial lipaemia is an established risk factor for atherosclerosis. To investigate the acute effect of four milk-derived dietary proteins (alpha-lactalbumin, whey isolate, caseinoglycomacropeptide and whey hydrolysate) on postprandial lipaemia, we have conducted a randomized, acute, single-blinded clinical intervention study with crossover design.

Subjects/Methods:

A total of 11 obese non-diabetic subjects (age: 44–74, BMI: 30–41.4 kg m-2) were included. On 4 different days the subjects ingested a high-fat meal with the following energy distribution: 66% energy from fat (100 g of butter), 15% of energy from carbohydrate (90 g of white wheat bread) and 19% of energy from protein (45 g of pure protein). Our primary variable was plasma triglyceride measured in the 8-h postprandial period. Secondarily, retinyl palmitate, non-esterified free fatty acids, glucose, insulin, glucagon, GLP-1 and GIP, active and total grehlin and cholecystokinin were measured.

Results:

We observed no statistically significant (P=0.8) differences between meals on our primary variable that is, triglycerides. Whey hydrolysate was associated with a significantly (P=0.02) smaller postprandial suppression of non-esterified free fatty acids compared with the other dietary proteins.

Conclusion:

We did not observe significant differences in postprandial lipaemia to the four milk-derived dietary proteins. Whey hydrolysate caused less postprandial suppression of free fatty acids.

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Acknowledgements

We wish to thank Tove Skrumsager and Lene Trudsø for excellent technical assistance. This work is carried out as a part of the research program of the Danish Obesity Research Centre (DanORC, see http://www.danorc.dk) and is supported by Nordic Centre of Excellence (NCoE) programme (Systems biology in controlled dietary interventions and cohort studies—SYSDIET, P number, 070014). Supported by ‘Marie Krogh Center for Metabolic Research’, Life Sciences, Copenhagen University. Supported by a grant from Arla Foods Ingredients amba, Viby, Denmark.

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Authors and Affiliations

  1. Department of Endocrinology and Metabolism MEA, Aarhus University Hospital, Aarhus, Denmark

    J Holmer-Jensen, M L Hartvigsen, L S Mortensen, C Thomsen & K Hermansen

  2. Department of Human Nutrition, Faculty of Life Sciences, University of Copenhagen, Frederiksberg C, Denmark

    A Astrup

  3. Federal Research Institute for Nutrition and Food, Institute for Physiology and Biochemistry of Nutrition, Kiel, Germany

    M de Vrese

  4. Department of Biomedical Sciences, The Panum Institute, University of Copenhagen, Copenhagen, Denmark

    J J Holst

Authors
  1. J Holmer-Jensen
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  2. M L Hartvigsen
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  4. A Astrup
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Correspondence to J Holmer-Jensen.

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Competing interests

J Holmer-Jensen, LS Mortensen and K Hermansen received a shared research grant from Arla. A Astrup is a scientific member of Global Dairy Platform (Chicago) and has received speaker's honoraria and research funding from the Danish Dairy Foundation, Arla and Danish Meat Association. None of the other authors had a conflict of interest to disclose.

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Holmer-Jensen, J., Hartvigsen, M., Mortensen, L. et al. Acute differential effects of milk-derived dietary proteins on postprandial lipaemia in obese non-diabetic subjects. Eur J Clin Nutr 66, 32–38 (2012). https://doi.org/10.1038/ejcn.2011.142

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