Abstract
Background/Objectives:
Free amino acids affect food palatability. As information on amino acids in frequently purchased pre-packaged food is virtually absent, we analyzed free amino acid patterns of 17 frequently purchased ready-to-serve convenience food products, and compared them with the information obtained from the respective food labels.
Subjects/Methods:
Quantitative amino acid analysis was performed using ion-exchange chromatography. γ-Aminobutyric acid (GABA) concentrations were verified using a stable isotope dilution gas chromatography/mass spectrometry (GC-MS) method. The patterns of free amino acids were compared with information obtained from food labels.
Results:
An obvious mismatch between free amino acid patterns and food label information was detected. Even on considering that tomatoes and cereal proteins are naturally rich in glutamate, the concentrations of free glutamate outranged the natural concentration of this amino acid in several products, and strongly suggested artificial enrichment. Free glutamate was found to be elevated even in dishes that explicitly state ‘no glutamate added’. Arginine was markedly elevated in lentils. Free cysteine was generally low, possibly reflecting thermal destruction of this amino acid during food processing. The meat and brain-specific dipeptide carnosine (CARN) was present in most meat-containing products. Some products did not contain detectable amounts of CARN in spite of meat content being claimed on the food labels. We detected GABA at concentrations that contribute significantly to the taste sensation.
Conclusion:
This investigation highlights a marked mismatch between food label information and food composition.
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References
Alexy U, Sichert-Hellert W, Rode T, Kersting M (2008). Convenience food in the diet of children and adolescents: consumption and composition. Br J Nutr 99, 345–351.
Bellisle F (2008). Experimental studies of food choices and palatability responses in European subjects exposed to the umami taste. Asia Pac J Clin Nutr 17, 376–379.
Beyreuther K, Biesalski HK, Fernstrom JD, Grimm P, Hammes WP, Heinemann U et al. (2007). Consensus meeting: monosodium glutamate—an update. Eur J Clin Nutr 61, 304–313.
Carratù B, Boniglia C, Giammarioli S, Mosca M, Sanzini E (2008). Free amino acids in botanicals and botanical preparations. J Food Sci 73, C323–C328.
Duva MA, Tomkins EM, Moranda LM, Kaplan R, Sukhaseum A, Bernardo JP et al. (2002). Regional differences in feeding and other behaviors elicited by N-methyl-D-aspartic acid in the rodent hypothalamus: a reverse microdialysis mapping study. Brain Res 925, 141–147.
Duva MA, Tomkins EM, Moranda LM, Kaplan R, Sukhaseum A, Stanley BG (2005). Origins of lateral hypothalamic afferents associated with N-methyl-d-aspartic acid-elicited eating studied using reverse microdialysis of NMDA and Fluorogold. Neurosci Res 52, 95–106.
Finlayson G, King N, Blundell JE (2007). Is it possible to dissociate ′liking′ and ′wanting′ for foods in humans? A novel experimental procedure. Physiol Behav 90, 36–42.
German Ministry of Food, Agriculture and Consumer Protection (2008): http://www.verbraucherschutz.sachsen-anhalt.de/wirueberuns/jahresberichte/jb04/jb2004-03-00.pdf (accessed 22 September 2008).
Halpern BP (2000). Glutamate and the flavor of foods. J Nutr 130 (4S Suppl), 910S–914S.
Hartmann BM, Bell S, Vasquez-Caicedo AL, Götz A, Erhardt J (2005) et al. Bundeslebensmittelschlüssel (German nutritional tables). Version II 3 1 Bundesforschungsanstalt für Ernährung und Lebensmittel (Federal Research Institute for Nutrition and Food). Karlsruhe (http://www.blsdb.de/uploads/media/BLS_Lizenzmodell_Bestellformular.pdf).
He K, Zhao L, Daviglus ML, Dyer AR, Van Horn L, Garside D, et al., INTERMAP Cooperative Research Group. (2008) Association of monosodium glutamate intake with overweight in Chinese adults: the INTERMAP Study. Obesity 6, 1875–1880.
Hermanussen M, García AP, Sunder M, Voigt M, Salazar V, Tresguerres JAF (2006). Obesity, voracity and short stature: the impact of glutamate on the regulation of appetite. Eur J Clin Nutr 60, 25–31.
Hermanussen M, Tresguerres JAF (2007). Overweight, appetite control, and the role of glutamate and excess nutritional protein during child development. Hum Ontogenet 1, 23–35.
Hisaki K, Wada K, Shinohara K, Nakamura Y, Ueno H (2008). The Effect of GABA on Human Taste Sensations and the Influence of Food Components on the activity of glutamate decarboxylase, GABA Synthesizing Enzyme. International Symposium on Olfaction and Taste: San Francisco, CA. (21–26 July, Abstract P346).
Hsieh YH, Ofori JA (2007). Innovations in food technology for health. Asia Pac J Clin Nutr 16 (Suppl 1), 65–73.
http://ep.espacenet.com/quickSearch?locale=en_EP (accessed 22 September 2008).
Jeng KC, Chen CS, Fang YP, Hou RC, Chen YS (2007). Effect of microbial fermentation on content of statin, GABA, and polyphenols in Pu-Erh tea. J Agric Food Chem 55, 8787–8792.
Kok RM, Howells DW, van den Heuvel CC, Guerand WS, Thompson GN, Jakobs C (1993). Stable isotope dilution analysis of GABA in CSF using simple solvent extraction and electron-capture negative-ion mass fragmentography. J Inherit Metab Dis 16, 508–512.
Louis-Sylvestre J, Giachetti I, Le Magnen J (1984). Sensory versus dietary factors in cafeteria-induced overweight. Physiol Behav 32, 901–905.
Maehashi K, Matsuzaki M, Yamamoto Y, Udaka S (1999). Isolation of peptides from an enzymatic hydrolysate of food proteins and characterization of their taste properties. Biosci Biotechnol Biochem 63, 555–559.
Makino Y, Soga N, Oshita S, Kawagoe Y, Tanaka A (2008). Stimulation of gamma-aminobutyric acid production in vine-ripe tomato (Lycopersicon esculentum mill fruits under modified atmospheres. J Agric Food Chem 56, 7189–7193.
Mattes RD (2008). Food palatability, rheology, and meal patterning. J Parenter Enteral Nutr 32, 572–574.
Obata H, Shimada K, Sakai N, Saito N (1997). GABAergic neurotransmission in rat taste buds: immunocytochemical study for GABA and GABA transporter subtypes. Brain Res Mol Brain Res 49, 29–36.
Park KB, Oh SH (2007). Production of yogurt with enhanced levels of gamma-aminobutyric acid and valuable nutrients using lactic acid bacteria and germinated soybean extract. Bioresour Technol 98, 1675–1679.
Rizzello CG, Cassone A, Di Cagno R, Gobbetti M (2008). Synthesis of angiotensin I-converting enzyme (ACE)-inhibitory peptides and gamma-aminobutyric acid (GABA) during sourdough fermentation by selected lactic acid bacteria. J Agric Food Chem 56, 6936–6943.
Rogers PJ, Blundell JE (1984). Meal patterns and food selection during the development of obesity in rats fed a cafeteria diet. Neurosci Biobehav Rev 8, 441–453.
Stanley BG, Willett VL, Donias HW, Dee MG, Duva MA (1996). Lateral hypothalamic NMDA receptors and glutamate as physiological mediators of eating and weight control. Am J Physiol 270 (2 Pt 2), R443–R449.
Stapleton JR, Roper SD, Delay ER (1999). The taste of monosodium glutamate (MSG), L-aspartic acid, and N-methyl-D-aspartate (NMDA) in rats: are NMDA receptors involved in MSG taste? Chem Senses 24, 449–457.
Walker R, Lupien JR (2000). The safety evaluation of monosodium glutamate. J Nutr 130 (4S Suppl), 1049S–1052S.
Acknowledgements
We thank Gesellschaft für Konsumforschung, Nuremburg, Germany. This study was supported by Deutsche Gesellschaft für Auxologie, and Norddeutsches Zentrum für Wachstumsforschung.
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Hermanussen, M., Gonder, U., Jakobs, C. et al. Patterns of free amino acids in German convenience food products: marked mismatch between label information and composition. Eur J Clin Nutr 64, 88–98 (2010). https://doi.org/10.1038/ejcn.2009.116
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DOI: https://doi.org/10.1038/ejcn.2009.116
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