Abstract
Guanosine and deoxyguanosine are detected in urine of PNP-deficient patients (1). We have studied the effects of these compounds on 3H-thymidine incorporation in human peripheral blood lymphocytes and malignant lymphoid cell lines. Peripheral blood lymphocytes, isolated by Percoll gradient centrifugation and counterflow elutriation were stimulated with various mitogens (PHA, Con A, PWM, SpA, StA) known to activate different lymphocyte subpopulations.
In all cell cultures tested deoxyguanosine inhibited 3H-thymidine more strongly than guanosine. Stimulated lymphocytes and cell lines were cultured in presence of different concentrations of guanosine and deoxyguanosine. Lymphocytes stimulated with B cell mitogens were as sensitive to guanosine and deoxyguanosine as lymphocytes stimulated with T cell mitogens were. Incorporation of 3H-thymidine in the cultured cell line Molt 4 by guanosine and deoxyguanosine was inhibited more severe than in the cultured RAJI (B) and REH (nonBnonT) cell lines and in stimulated peripheral blood lymphocytes. When possibilities for antileukemic chemotherapy using deoxyguanosine or guanosine are considered, deoxyguanosine seems to be a better candidate than guanosine.
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Van Laarhoven, J., Van Der Kraan, P. EFFECTS OF GUANOSINE AW DEOXYGUANOSINE W 3H-THYMIDINE INCORPORATION OF HUMAN LYHPHOID CELLS: 220. Pediatr Res 19, 780 (1985). https://doi.org/10.1203/00006450-198507000-00240
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DOI: https://doi.org/10.1203/00006450-198507000-00240