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Characterization and biological significance of IL-23-induced neutrophil polarization

Cellular & Molecular Immunology volumeĀ 15,Ā pages 518ā€“530 (2018)Cite this article

Abstract

Neutrophils are heterogeneous with distinct subsets, and can switch phenotypes to exert regulatory functions on immunity. We herein demonstrate that IL-23-treated neutrophils selectively produce IL-17A, IL-17F and IL-22, and display a distinct gene expression profile in contrast to resting and lipopolysaccharide-treated neutrophils. IL-17+ neutrophils are present in the colons of mice with dextran sulfate sodium-induced colitis. Adoptive transfer of IL-23-treated neutrophils significantly promotes pathogenesis in this model. IL-23 induces neutrophil polarization through STAT3-dependent RORĪ³t and BATF pathways. Thus, IL-23-induced neutrophil polarization expresses a unique cytokine-producing profile, which may contribute to IL-23-mediated inflammatory diseases.

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Acknowledgements

We thank Dr Aqeel Javeed for his kind review of the manuscript. This work was supported by grants from the National Basic Research Program of China (2014ZX10002002-001-002, YZ; 2011CB710903, YZ), the National Natural Science Foundation of China for General and Key Programs (81530049, 81130055, YZ), the Knowledge Innovation Program of the Chinese Academy of Sciences (XDA04020202-19, YZ), the Beijing Municipal Hospital Authority ā€˜Yangfan Programā€™ (ZYLX201408, XZ), and the CAS/SAFEA International Partnership Program for Creative Research Teams (YZ).

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  1. These authors contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, 100020, Beijing, China

    Yang Li,Ā Linnan Zhu,Ā Zhulang Chu,Ā Tao Yang,Ā Hai-Xi Sun,Ā Fan Yang,Ā Yuzhu Hou,Ā Peng Wang,Ā Qingjie Zhao,Ā Yaling TaoĀ &Ā Yong Zhao

  2. University of Chinese Academy of Sciences, 100020, Beijing, China

    Yang LiĀ &Ā Zhulang Chu

  3. Department of Urology, Beijing Chaoyang Hospital, Capital Medical University, 100020, Beijing, China

    Wei WangĀ &Ā Xiaodong Zhang

  4. Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Peking Union Medical College, 100021, Beijing, China

    Lianfeng Zhang

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Contributions

YL, LZ, ZC and TY designed and carried out the experiments, analyzed the data and wrote the manuscript; H-XS analyzed the microarray data; FY, WW and YH performed the animal model experiments and real-time PCR assays; PW performed the ELISA assays; QZ and YT performed the flow cytometry; LZ, XZ and YZ designed the experiments, analyzed the data, wrote the manuscript and provided overall supervision.

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Correspondence to Lianfeng Zhang, Xiaodong Zhang or Yong Zhao.

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Li, Y., Zhu, L., Chu, Z. et al. Characterization and biological significance of IL-23-induced neutrophil polarization. Cell Mol Immunol 15, 518ā€“530 (2018). https://doi.org/10.1038/cmi.2017.39

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