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Type I and II interferons enhance dendritic cell maturation and migration capacity by regulating CD38 and CD74 that have synergistic effects with TLR agonists

Cellular & Molecular Immunology volume 8, pages 341–347 (2011)Cite this article

Abstract

The major limitation for the maturation of dendritic cells (DCs) using Toll-like receptor (TLR) agonists is their decreased ability to migrate into lymph nodes compared with conventional DCs. CD38 can be used as a multifunctional marker to modulate migration, survival and Th1 responses of DCs. CD74 has been shown to negatively regulate DC migration. The goal of this study was to investigate the combinations of TLR agonists and interferons (IFNs) that most effectively regulate CD38 and CD74 expression on DCs. Synergistic TLR agonist stimulation in combination with IFN-α and IFN-γ was the best method for regulating CD38 and CD74 expression and inducing the highest secretion of IL-12p70. An in vitro migration assay showed that DCs treated with this combination had significantly enhanced migratory ability, similar to that observed in cells expressing CD38, CD74 and CCR7. The results of this study suggest that an alternative maturation protocol in which two TLR ligands are combined with type I and II IFNs generates potent DCs that have both a high migratory capacity and high IL-12p70 production.

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Acknowledgements

This study was financially supported by a grant from the Korea Healthcare Technology R&D Project (A080489), Ministry of Health & Welfare, Korea, and by Grant No. RTI05-01-01 from the Regional Technology Innovation Program of the Ministry of Commerce, Industry and Energy, Korea.

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Author notes

  1. Dr JJ Lee, Department of Hematology-Oncology, Chonnam National University Hwasun Hospital, 160 Ilshimri, Hwasun, Jeollanamdo 519-763, Korea. E-mail: drjejung@chonnam.ac.kr

Authors and Affiliations

  1. Research Center for Cancer Immunotherapy, Chonnam National University Hwasun Hospital, Jeollanamdo, Korea

    Thanh-Nhan Nguyen-Pham, Mi-Seon Lim, Truc Anh Thi Nguyen, Youn-Kyung Lee, Chun-Ji Jin, Hyun Ju Lee, Cheol Yi Hong, Jae-Sook Ahn, Deok-Hwan Yang, Ik-Joo Chung & Je-Jung Lee

  2. Department of Hematology-Oncology, Chonnam National University, Gwangju, Korea

    Thanh-Nhan Nguyen-Pham, Jae-Sook Ahn, Deok-Hwan Yang, Yeo-Kyeoung Kim, Ik-Joo Chung, Hyeoung-Joon Kim & Je-Jung Lee

  3. The Brain Korea 21 Project, Center for Biomedical Human Resources at Chonnam National University, Gwangju, Korea

    Mi-Seon Lim, Truc Anh Thi Nguyen, Chun-Ji Jin, Ik-Joo Chung & Je-Jung Lee

  4. Vaxcell-Bio Therapeutics, Jeollanamdo, Korea

    Youn-Kyung Lee & Je-Jung Lee

  5. Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Korea

    Byoung Chul Park

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Nguyen-Pham, TN., Lim, MS., Nguyen, T. et al. Type I and II interferons enhance dendritic cell maturation and migration capacity by regulating CD38 and CD74 that have synergistic effects with TLR agonists. Cell Mol Immunol 8, 341–347 (2011). https://doi.org/10.1038/cmi.2011.7

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