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Cell Selection

The combination method using magnetic beads and a magnet helps sustain the number of donor BM cells after intra-BM injection, resulting in rapid hematopoietic recovery

Bone Marrow Transplantation volume 45, pages 993–999 (2010)Cite this article

Abstract

We have developed a new BMT method, intra-BM-BMT (IBM-BMT), in which donor BM cells (BMCs) are directly injected into the recipient’s BM, resulting in a rapid recovery of donor hematopoiesis and a reduction in the severity of GVHD. In the present experiment, we attempted to retain the number of injected BMCs using magnetic beads and a magnet. The BMCs of donor mice were conjugated with magnetic beads, and these cells were then injected into the BM of recipient mice with a magnet (magnet-IBM group) and compared with conventional IBM-BMT without a magnet (IBM group). A significantly higher number of transplanted cells were detected in the injected BM in the magnet-IBM group. We next carried out day-12 colony-forming units of spleen (CFU-S) assays to examine the early stage of hematopoiesis of the injected host hematopoietic stem cells after IBM-BMT. The spleens of mice in the magnet-IBM group showed considerably higher CFU-S counts than those in the IBM group. Excellent reconstitution of donor hematopoietic cells in the magnet-IBM group was observed 1 month after IBM-BMT. These results suggest that the IBM-BMT using the combination of magnetic beads and a magnet is superior to the conventional IBM-BMT.

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Acknowledgements

We thank Ms Y Tokuyama, Ms K Hayashi and Ms A Kitajima for their expert technical assistance, and also Mr Hilary Eastwick-Field and Ms K Ando for the preparation of this manuscript. This work was supported by a grant from the ‘The 21st Century COE Program’ of the Ministry of Education, Culture, Sports, Science and Technology, the Department of Transplantation for Regeneration Therapy (sponsored by Otsuka Pharmaceutical Company, Ltd), Molecular Medical Science Institute, Otsuka Pharmaceutical Co., Ltd, Japan Immunoresearch Laboratories Co., Ltd (JIMRO), Scientific Research (C) 21590447 and an award from Dr Toshiko Kitanishi.

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Authors and Affiliations

  1. First Department of Pathology, Kansai Medical University, Osaka, Japan

    C Shima, Y Adachi, M Shi, Y Imai, S Yanai & S Ikehara

  2. Department of Ophthalmology, Kansai Medical University, Osaka, Japan

    C Shima, K Minamino & K Takahashi

  3. Transplantation Center, Kansai Medical University, Osaka, Japan

    Y Adachi & S Ikehara

  4. Regeneration Research Center for Intractable Diseases, Kansai Medical University, Osaka, Japan

    Y Adachi, K Takahashi & S Ikehara

  5. Department of Cardiovascular Medicine, Kyoto Prefectural University School of Medicine, Kyoto, Japan

    M Okigaki

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  1. C Shima
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Correspondence to S Ikehara.

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Shima, C., Adachi, Y., Shi, M. et al. The combination method using magnetic beads and a magnet helps sustain the number of donor BM cells after intra-BM injection, resulting in rapid hematopoietic recovery. Bone Marrow Transplant 45, 993–999 (2010). https://doi.org/10.1038/bmt.2009.278

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