Abstract
Conditioned media from 14 short term fibroblast cell lines were mitogenic for human breast cancer cells with different steroid receptor profiles in serum-free culture. Fibroblast-conditioned medium stimulated tritiated thymidine incorporation in short term culture and growth in a longer proliferation study as measured by the MTT colorimetric assay. Conditioned media from benign and malignant epithelial cells were non-stimulatory for breast cancer cells but that derived from endothelial cells showed similar stimulation to fibroblasts. Partial purification of fibroblast-conditioned medium identified a peptide with a molecular weight of approximately 8 kDa that showed no affinity for heparin and was mitogenic for MCF-7 breast cancer cells.
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Ryan, M., Orr, D. & Horgan, K. Fibroblast stimulation of breast cancer cell growth in a serum-free system. Br J Cancer 67, 1268–1273 (1993). https://doi.org/10.1038/bjc.1993.237
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DOI: https://doi.org/10.1038/bjc.1993.237
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