Abstract
The proliferation rate of non-Hodgkin's lymphomas (NHL) was estimated by using 3 different methods. In cell suspension we determined the proportion of cells in cycle with the monoclonal antibody (Mab) Ki-67 and also in S-phase after the incorporation of bromo-deoxyuridine (BrdU) utilizing Mab anti-BrdU. In low grade lymphomas 3.5 +/- 1.6% of the cells were in cycle and 1.2 +/- 0.9% in S-phase, the corresponding values for high grade lymphomas were 22.5 +/- 18.7% and 8.9 +/- 7.8% respectively. Frozen sections of NHL were reacted with an antibody to the transferrin receptor (TR) and Ki67 as markers for proliferative activity. A high number of TR positive cells was found in low grade lymphomas of all histological types, whereas Ki67 positivity correlated closely with grading. With a few exceptions, low grade lymphomas contained less than 25% Ki67 positive cells within the tumour cell population. This observation is relevant to treatment strategies for low grade NHL.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 24 print issues and online access
$259.00 per year
only $10.79 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
Rights and permissions
About this article
Cite this article
Schrape, S., Jones, D. & Wright, D. A comparison of three methods for the determination of the growth fraction in non-Hodgkin's lymphoma. Br J Cancer 55, 283–286 (1987). https://doi.org/10.1038/bjc.1987.54
Issue Date:
DOI: https://doi.org/10.1038/bjc.1987.54
This article is cited by
-
Comparison of immunostainings for proliferating cell nuclear antigen and Ki-67 in human extraocular lesions
Graefes Archive for Clinical and Experimental Ophthalmology (1997)
-
The correlation of Ki-67 staining indices with tumour doubling times in regrowing non-functioning pituitary adenomas
Acta Neurochirurgica (1996)
-
FDG-PET for predicting the prognosis of malignant lymphoma
Annals of Nuclear Medicine (1994)
-
The significance of Ki-67, BrdU and OKT9 expression in determination of myelodysplastic syndromes by use of APAAP technique
Journal of Tongji Medical University (1993)
-
The assessment of cellular proliferation by immunohistochemistry: A review of currently available methods and their applications
The Histochemical Journal (1992)