Abstract
The reactivation of U.V.-irradiated adenovirus 2 in HeLa cells is enhanced 8-9 fold if the cells are given a brief hyperthermic shock before infection. Maximum reactivation is achieved by heating for 10 min at 45.5 degrees C and with a delay of 36 h between heating and infection. The induction process requires protein synthesis only during the 3 h period immediately following heating, cycloheximide does not prevent the expression of enhanced reactivation if added to the cells after this time. Heat-enhanced reactivation exhibits properties similar in some respects to radiation-enhanced reactivation and indicates an increased capacity of the heated cells to tolerate DNA damage.
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Piperakis, S., McLennan, A. Hyperthermia enhances the reactivation of irradiated adenovirus in HeLa cells. Br J Cancer 49, 199–205 (1984). https://doi.org/10.1038/bjc.1984.32
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DOI: https://doi.org/10.1038/bjc.1984.32