Intense tumour-cell destruction by syngeneic mice: role of macrophages, complement activation and tumour-cell factors

Abstract

When injected i.p. and in large numbers (10(7)) into syngeneic mice, 125IUdR-labelled L1210 cells are rapidly destroyed in a small proportion of animals, while in the other animals the lysis is low. This bimodal distribution is clearly visible 24 h after cell injection. The intense lysis occurs in fewer animals when macrophage-derived lysosomal enzymes are inhibited by trypan blue and if the complement is depleted by high doses of cobra venom factor (CVF). The intense destruction occurs in more animals after adjuvant treatment, if the mice are latently contaminated, after a moderate production of C3b by low doses of CVF, or after the injection of a tumour-cell dialysate. The destruction seems to be the result of positive feedback reaction which involves at least macrophages and complement activation.