Abstract
Chalone prepared from primary BALB/c mouse embryo fibroblasts caused a 33% reduction in incorporation of tritiated thymidine in the cultures of the second in vitro passage of BALB/c embryo fibroblasts, whereas chalone prepared from thymus, skin and spleen was without effect. Pretreatment of BALB/c secondary fibroblasts with the polyanions dextran sulphate and heparin abrogated the chalone effect. The polycations DEAE-dextran and polybrene were without effect. The effect of incubation with the dextran sulphate polyanion was reversed when followed by incubation with DEAE-dextran polycation.
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Ebbesen, P., Olsson, L. Chalone-like effect abrogated by dextran sulphate and heparin polyanion pretreatment of target cells. Br J Cancer 31, 649–652 (1975). https://doi.org/10.1038/bjc.1975.111
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DOI: https://doi.org/10.1038/bjc.1975.111
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