Abstract
Aim:
To explore the anti-inflammatory effects of a novel peptide designed to bind with the NF-κB p50 subunit.
Methods:
The affinity of the peptide binding with p50 was measured with a biosensor. Levels of tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6) from a human acute monocytic leukemia cell line (THP-1) treated with lipopolysaccharide (LPS) were measured using the ELISA method. In vivo anti-inflammatory effects of the peptide were tested with phorbol myristate acetate (PMA)-induced ear edema and zymosan A-induced peritonitis in mice.
Results:
The peptide has the ability to interact with the NF-κB p50 subunit and can effectively inhibit TNF-α and IL-6 production in the THP-1 cell line, PMA-induced ear edema and zymosan A-induced peritonitis in mice.
Conclusion:
The peptide may have therapeutic potential for the treatment of local acute inflammation.
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Project supported by the National Natural Science Foundation of China (No 30200270 and 30400569).
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Wang, Zp., Cai, Sx., Liu, Db. et al. Anti-inflammatory effects of a novel peptide designed to bind with NF-κB p50 subunit. Acta Pharmacol Sin 27, 1474–1478 (2006). https://doi.org/10.1111/j.1745-7254.2006.00442.x
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DOI: https://doi.org/10.1111/j.1745-7254.2006.00442.x
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