Abstract
Aim:
To observe the effects of Ginkgo biloba extract (EGb) on the hypertrophy of mesangial cells and the accumulation of extracellular matrix (ECM) in mesangial cells.
Methods:
Cultured mesangial cells were allotted into 7 groups: normal group, solvent control group, high glucose group, low dose of EGb group, moderate dose of EGb group, high dose of EGb group, and captopril group. Activities of cell antioxidases, S phase percentage and G0/G1 phase percentage, collagen IV and laminin, Smad2/3 and Smad7, TGF-β1 mRNA were measured by different methods.
Results:
For EGb-treated groups, when compared with high glucose group, the cell percentage of S phase was raised and the percentage of G0/G1 was lowered. The intensity of oxidative stress was weakened. The expression of Smad2/3 was greatly decreased and Smad7 was increased. Collagen IV, laminin and TGF-β1 mRNA were also reduced.
Conclusion:
EGb can suppress cell hypertrophy and the accumulation of ECM in rat mesangial cells, which means it could play a vital role in the delay of glomerulosclerosis in diabetic nephropathy.
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Project supported by College Natural Science Research Foundation of Jiangsu Province (No 03KJ13360143).
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Wang, Jy., Yin, Xx., Wu, Ym. et al. Ginkgo biloba extract suppresses hypertrophy and extracellular matrix accumulation in rat mesangial cells. Acta Pharmacol Sin 27, 1222–1230 (2006). https://doi.org/10.1111/j.1745-7254.2006.00360.x
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DOI: https://doi.org/10.1111/j.1745-7254.2006.00360.x
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