Abstract
Aim:
To investigate the inductive effect of water extract from Hericium erinaceum (WEHE) on interleukin-1 β (IL-1β) expression.
Methods:
A murine macrophage cell-line, RAW 264.7 was stimulated with 1 to 10 mg/L WEHE and inductions of IL-1β protein and its steady state mRNA were examined using a bioassay, Western blotting, and reverse transcription-polymerase chain reaction (RT-PCR) analysis. The inductive effect of WEHE on IL-1β gene expression was further investigated by a chloramphenicol acetyltransferase (CAT) reporter gene assay using a transient transfection with pIL-1(870 bp)-CAT where the expression of the CAT gene was regulated by a IL-1β promoter. An electrophoretic mobility shift assay (EMSA) was also performed to examine transcription factors, nuclear factor-kappa B (NF-κB), activator protein 1 (AP-1), nuclear factor interleukin-6 (NF-IL6), and cAMP response element (CRE)/activating transcription factor (ATF).
Results:
WEHE induced IL-1β production in both its mRNA and protein expression in a dose-dependent manner. The inductive effect of WEHE on IL-1β gene expression was due to the augmentation of the IL-1β transcription. Furthermore, EMSA showed that WEHE markedly increased the binding activities of NF-κB, and to a lesser extent, those of AP-1 and NF-IL6 to their cognate DNA recognition sites, whereas CRE/ATF binding remained constant, all of which are known to be involved in the regulation of IL-1β gene expression.
Conclusion:
WEHE induces IL-1β expression in macrophages at a transcriptional level by enhancing the activation of transcription factors, NF-κB, NF-IL6, and AP-1.
Similar content being viewed by others
Article PDF
References
Zaidman BZ, Yassin M, Mahajna J, Wasser SP . Medicinal mushroom modulators of molecular targets as cancer therapeutics. Appl Microbiol Biotechnol 2005; 67: 453–68.
Carrizo ME, Capaldi S, Perduca M, Irazoqui FJ, Nores GA, Monaco HL . The antineoplastic lectin of the common edible mushroom (Agaricus bisporus) has two binding sites, each specific for a different configuration at a single epimeric hydroxyl. J Biol Chem 2005; 280: 10614–23.
de Vere White RW, Hackman RM, Soares SE, Beckett LA, Sun B . Effects of a mushroom mycelium extract on the treatment of prostate cancer. Urology 2002; 60: 640–4.
Chang R . Functional properties of edible mushrooms. Nutr Rev 1996; 54: S91–3.
Ko HG, Park SH, Kim SH, Park HG, Park WM . Detection and recovery of hydrolytic enzymes from spent compost of four mushroom species. Folia Microbiol (Praha) 2005; 50: 103–6.
Wang HX, Ng TB . A new laccase from dried fruiting bodies of the monkey head mushroom Hericium erinaceum. Biochem Biophys Res Commun 2004; 322: 17–21.
Kawagishi H, Mori H, Uno A, Kimura A, Chiba S . A sialic acid-binding lectin from the mushroom Hericium erinaceum. FEBS Lett 1994; 340: 56–8.
Nakatsugawa M, Takahashi H, Takezawa C, Nakajima K, Harada K, Sugawara Y, et al. Hericium erinaceum (yamabushitake) extract-induced acute respiratory distress syndrome monitored by serum surfactant proteins. Intern Med 2003; 42: 1219–22.
Lee EW, Shizuki K, Hosokawa S, Suzuki M, Suganuma H, Inakuma T, et al. Two novel diterpenoids, erinacines H and I from the mycelia of Hericium erinaceum. Biosci Biotechnol Biochem 2000; 64: 2402–5.
Kenmoku H, Shimai T, Toyomasu T, Kato N, Sassa T . Erinacine Q, a new erinacine from Hericium erinaceum, and its biosyn-thetic route to erinacine C in the basidiomycete. Biosci Biotechnol Biochem 2002; 66: 571–5.
Mizuno T, Wasa T, Ito H, Suzuki C, Ukai N . Antitumor-active polysaccharides isolated from the fruiting body of Hericium erinaceum, an edible and medicinal mushroom called yamabushitake or houtou. Biosci Biotechnol Biochem 1992; 56: 347–8.
Rossi B . IL-1 transduction signals. Eur Cytokine Netw 1993; 4: 181–7.
Dayer JM . Evidence for the biological modulation of IL-1 activity: the role of IL-1Ra. Clin Exp Rheumatol 2002; 20: S14–20.
Khoruts A, Osness RE, Jenkins MK . IL-1 acts on antigen-presenting cells to enhance the in vivo proliferation of antigen-stimulated naive CD4 T cells via a CD28-dependent mechanism that does not involve increased expression of CD28 ligands. Eur J Immunol 2004; 34: 1085–90.
Wesa A, Galy A . Increased production of pro-inflammatory cytokines and enhanced T cell responses after activation of human dendritic cells with IL-1 and CD40 ligand. BMC Immunol 2002; 3: 14.
Nakae S, Asano M, Horai R, Sakaguchi N, Iwakura Y . IL-1 enhances T cell-dependent antibody production through induction of CD40 ligand and OX40 on T cells. J Immunol 2001; 167: 90–7.
Chelvarajan RL, Gilbert NL, Bondada S . Neonatal murine B lymphocytes respond to polysaccharide antigens in the presence of IL-1 and IL-6. J Immunol 1998; 161: 3315–24.
Dinarello CA . The IL-1 family and inflammatory diseases. Clin Exp Rheumatol 2002; 20: S1–13.
Calkins CM, Bensard DD, Shames BD, Pulido EJ, Abraham E, Fernandez N, et al. IL-1 regulates in vivo C-X-C chemokine induction and neutrophil sequestration following endotoxemia. J Endotoxin Res 2002; 8: 59–67.
Yamada T, Fujieda S, Yanagi S, Yamamura H, Inatome R, Yamamoto H, et al. IL-1 induced chemokine production through the association of Syk with TNF receptor-associated factor-6 in nasal fibroblast lines. J Immunol 2001; 167: 283–8.
Furutani Y . Molecular studies on interleukin-1 alpha. Eur Cytokine Netw 1994; 5: 533–8.
Uhing RJ, Adams DO . Molecular events in the activation of murine macrophages. Agents Actions 1989; 26: 9–14.
Gao B, Tsan MF . Induction of cytokines by heat shock proteins and endotoxin in murine macrophages. Biochem Biophys Res Commun 2004; 317: 1149–54.
Schilling D, Beissert T, Fenton MJ, Nixdorff K . Negative regulation of IL-1beta production at the level of transcription in macrophages stimulated with LPS. Cytokine 2001; 16: 51–61.
Kang JS, Jeon YJ, Suh J, Park SK, Yang KH, Kim HM . 2-Acetylaminofluorene inhibits interleukin-1beta production in LPS-stimulated macrophages by blocking NF-kappaB/Rel activation. Cancer Lett 2004; 203: 91–8.
Goto M, Katayama KI, Shirakawa F, Tanaka I . Involvement of NF-kappaB p50/p65 heterodimer in activation of the human pro-interleukin-1 beta gene at two subregions of the upstream enhancer element. Cytokine 1999; 11: 16–28.
Muller JM, Ziegler-Heitbrock HW, Baeuerle PA . Nuclear factor kappa B, a mediator of lipopolysaccharide effects. Immunobiology 1993; 187: 233–56.
Yang H, Tuzun E, Alagappan D, Yu X, Scott BG, Ischenko A, et al. IL-1 Receptor antagonist-mediated therapeutic effect in murine myasthenia gravis is associated with suppressed serum proinflammatory cytokines, C3, and anti-acetylcholine receptor IgG1. J Immunol 2005; 175: 2018–25.
Dinarello CA . Blocking IL-1 in systemic inflammation. J Exp Med 2005; 201: 1355–9.
Pascual V, Allantaz F, Arce E, Punaro M, Banchereau J . Role of interleukin-1 (IL-1) in the pathogenesis of systemic onset juvenile idiopathic arthritis and clinical response to IL-1 blockade. J Exp Med 2005; 201: 1479–86.
Braddock M, Quinn A . Targeting IL-1 in inflammatory disease: new opportunities for therapeutic intervention. Nat Rev Drug Discov 2004; 3: 330–9.
Association of Official Analytical Chemists. 15th ed. Washington, DC: Official Methods of Analysis; 1990.
Jeon YJ, Han SH, Lee YW, Lee M, Yang KH, Kim HM . Dexamethasone inhibits IL-1 beta gene expression in LPS-stimulated RAW 264.7 cells by blocking NF-kappa B/Rel and AP-1 activation. Immunopharmacology 2000; 48: 173–83.
Han SH, Yea SS, Jeon YJ, Yang KH, Kaminski NE . Transforming growth factor-beta 1 (TGF-beta1) promotes IL-2 mRNA expression through the up-regulation of NF-kappaB, AP-1 and NF-AT in EL4 cells. J Pharmacol Exp Ther 1998; 287: 1105–12.
Gorman CM, Moffat LF, Howard BH . Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol Cell Biol 1982; 2: 1044–51.
Hsieh TC, Chiao JW . Growth modulation of human prostatic cancer cells by interleukin-1 and interleukin-1 receptor antagonist. Cancer Lett 1995; 95: 119–23.
Kilian PL, Kaffka KL, Biondi DA, Lipman JM, Benjamin WR, Feldman D, et al. Antiproliferative effect of interleukin-1 on human ovarian carcinoma cell line (NIH: OVCAR-3). Cancer Res 1991; 51: 1823–8.
Paciotti GF, Tamarkin L . Interleukin-1 directly regulates hormone-dependent human breast cancer cell proliferation in vitro. Mol Endocrinol 1988; 2: 459–64.
Karin M, Delhase M . The I kappa B kinase (IKK) and NF-kappa B: key elements of proinflammatory signalling. Semin Immunol 2000; 12: 85–98.
Baeuerle PA, Henkel T . Function and activation of NF-kappa B in the immune system. Annu Rev Immunol 1994; 12: 141–79.
Siebenlist U, Franzoso G, Brown K . Structure, regulation and function of NF-kappa B. Annu Rev Cell Biol 1994; 10: 405–55.
May MJ, Ghosh S . Signal transduction through NF-kappa B. Immunol Today 1998; 19: 80–8.
Connelly L, Palacios-Callender M, Ameixa C, Moncada S, Hobbs AJ . Biphasic regulation of NF-kappa B activity underlies the pro- and anti-inflammatory actions of nitric oxide. J Immunol 2001; 166: 3873–81.
Author information
Authors and Affiliations
Corresponding author
Additional information
Project supported by a grant of the Oriental Medicine R&D Project (B050018), Ministry of Health & Welfare, Republic of Korea.
Rights and permissions
About this article
Cite this article
Son, Cg., Shin, Jw., Cho, Jh. et al. Induction of murine interleukin-1 beta expression by water-soluble components from Hericium erinaceum. Acta Pharmacol Sin 27, 1058–1064 (2006). https://doi.org/10.1111/j.1745-7254.2006.00321.x
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1111/j.1745-7254.2006.00321.x
Keywords
This article is cited by
-
Medicinal properties of Hericium erinaceus and its potential to formulate novel mushroom-based pharmaceuticals
Applied Microbiology and Biotechnology (2014)
-
Le Hericium erinaceus: des propriétés essentiellement dépendantes du neuronal growth factor
Phytothérapie (2011)