Abstract
Aim:
To study the effects of {2-[(3-carboxy-1-oxoprogy1)amino]-2-deoxy-D-glucose (COPADG) on cultured human hepatocellular carcinoma cells (HepG2).
Methods:
HepG2 cells were cultured in RPMI-1640 medium. Cell proliferation was determined by MTT assay. Apoptosis was determined by fluorescence microscopy, transmission electron microscopy, agarose gel electrophoresis of DNA fragmentation, and flow cytometry.
Results:
At the concentration ranging between 1–30 μmol/L, COPADG potently inhibited the growth and induced apoptosis of HepG2 cells.
Conclusion:
COPADG could effectively induce apoptosis in human hepatocellular carcinoma cells. More investigations are warranted for the potential use of this compound as a new agent for the non-surgical management of human hepatocellular carcinoma.
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Project supported by the Creative Engineering Realm the Item of Chinese Academia of Sciences, CX805.
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Wu, J., Kou, W., Gao, Mt. et al. Effects of {2-[(3-carboxy-1-oxoprogy1)amino]-2-deoxy-D-glucose} on human hepatocellular carcinoma cell line. Acta Pharmacol Sin 26, 635–640 (2005). https://doi.org/10.1111/j.1745-7254.2005.00066.x
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DOI: https://doi.org/10.1111/j.1745-7254.2005.00066.x