Abstract
Aim:
To examine the effect of daidzin, genistin, and glycitin on the osteogenic and adipogenic differentiation of bone marrow stromal cells (MSC) and the adipogenic transdifferentiation of osteoblasts.
Methods:
MTT test, alkaline phosphatase (ALP) activity measurement, Oil Red O stain and measurement were employed.
Results:
Daidzin, genistin, and glycitin 1×10-8, 5×10-7, 1×10-6, 5×10-6, and 1×10-5 mol/L all promoted the proliferation of primary mouse bone MSC and osteoblasts. Daidzin 5×10-7 mol/L and genistin 1×10-6 mol/L promoted the osteogenesis of MSC. Genistin 1×10-8, 5×10-7, 1×10-6, 5×10-6, and 1×10-5 mol/L and glycitin 1×10-8, 1×10-6, and 1×10-5 mol/L inhibited the adipogenesis of MSC. Daidzin, genistin, and glycitin 1×10-8, 5×10-7, 1×10-6, 5×10-6, and 1×10-5 mol/L all inhibited the adipocytic transdifferentiation of osteoblasts.
Conclusions:
Daidzin, genistin, and glycitin may modulate differentiation of MSC to cause a lineage shift toward the osteoblast and away from the adipocytes, and could inhibit adipocytic transdifferentiation of osteoblasts. They could also be helpful in preventing the development of osteonecrosis.
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Li, Xh., Zhang, Jc., Sui, Sf. et al. Effect of daidzin, genistin, and glycitin on osteogenic and adipogenic differentiation of bone marrow stromal cells and adipocytic transdifferentiation of osteoblasts. Acta Pharmacol Sin 26, 1081–1086 (2005). https://doi.org/10.1111/j.1745-7254.2005.00161.x
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DOI: https://doi.org/10.1111/j.1745-7254.2005.00161.x
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