Abstract
The carcinogenicity of the vegetative tissues of bracken fern (Pteridium) has long been established. More recently, the carcinogenic effects of the spores of bracken have also been recognized. Both vegetative tissues and spores of bracken can induce adducts in DNA in animal tissues, but the possible genotoxic or carcinogenic effects of spores from fern species other than bracken are unknown. The single-cell gel electrophoresis (‘comet’) assay was used to investigate whether fern spores can cause DNA damage in vitro. Extracts of spores from six fern species were administered to cultured human premyeloid leukaemia (K562) cells. Spore extracts of five fern species: Anemia phyllitidis, Dicksonia antarctica, Pteridium aquilinum, Pteris vittata and Sadleria pallida, induced significantly more DNA strand breaks than those in the control groups. Only in one species, Osmunda regalis, was the effect no different from that in the control groups. Using extracts from A. phyllitidis and P. vittata, the extent of DNA damage was increased by increasing the original dose 10 times, whereas an experiment in which exposure times were varied suggested that the highest levels of strand breaks appear after 2 h exposure. Simultaneous incubation with human S9 liver enzyme mix ablated the damaging effect of the extracts. Our data show that fern spore extracts can cause DNA damage in human cells in vitro. Considering the strong correlation between DNA damage and carcinogenic events, the observations made in this report may well have some implications for human health. © 2000 Cancer Research Campaign
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References
Alonso-Amelot ME, Castillo U, Smith BL and Lauren DR (1996) Bracken ptaquiloside in milk. Nature 382: 587
Anderson D, Yu T-W and McGregor DB (1998) Comet assay responses as indicators of carcinogen exposure. Mutagenesis 13: 539–555
Ashby J, Tinwell H, Lefevre PA and Browne MA (1995) The single cell gel electrophoresis assay for induced DNA damage (comet assay) measurement of tail length and moment. Mutagenesis 10: 85–90
Basaran AA, Yu T-W, Plewa MJ and Anderson D (1996) An investigation of some Turkish herbal medicines in Salmonella typhimurium and in the comet assay in human lymphocytes. Teratog Carcinog Mutagen 16: 125–138
Bringuier P-P, Piaton E, Berger N, Debruyne F, Perrin P, Schalken J and Devonec M (1995) Bracken fern-induced bladder tumors in guinea pigs. Am J Pathol 147: 858–868
Bunnag D, Dhorranintra B and Limsuvan S (1989) Ferns and their allergenic importance: skin and nasal provocation tests to fern spore extract in allergic and non-allergic patients. Annals of Allergy 62: 554–558
Buschfort C, Müller MR, Seeber S, Rajewsky MF and Thomale J (1997) DNA excision repair profiles of normal and leukemic human lymphocytes: functional analysis at the single-cell level. Cancer Res 57: 651–658
Cebulska-Wasilewska A, Nowak D, Niedzwiedz W and Anderson D (1998) Correlations between DNA and cytogenic damage induced after chemical treatment and radiation. Mutat Res 421: 83–91
Conway E (1952) Bracken – the problem plant. Scottish Agriculture Spring: 181–184
Evans IA (1987) Bracken carcinogenicity. In International quarterly scientific reviews, Reviews on environmental health, Vol. VII, James GV (ed) pp 161–199. Freund Publishing House Ltd: Tel-Aviv
Evans IA and Mason J (1965) Carcinogenic activity of bracken. Nature 208: 913–914
Fairbairn DW, Olive PL and O’Neill KL (1995) The comet assay: a comprehensive review. Mutat Res 339: 37–59
Fortini P, Raspaglio G, Falchi M and Dogliotti E (1996) Analysis of DNA alkylation damage and repair in mammalian cells by the comet assay. Mutagenesis 11: 169–175
Leroy T, Van Hummelen P, Anard D, Castelain P, Kirch-Volders M, Lauwerys R and Lison D (1996) Evaluation of three methods for the detection of DNA single-strand breaks in human lymphocytes: alkaline elution, nick translation, and single-cell gel electrophoresis. J Toxicol Environ Health 47: 409–422
Ojika M, Wakamatsu K, Niwa H and Yamada K (1987) Ptaquiloside, a potent carcinogen isolated from bracken fern Pteridium aquilinum var. latiusculum: structure elucidation based on chemical and spectral evidence and reactions with amino acids, nucleosides and nucleotides. Tetrahedron 43: 5261–5274
Östling O and Johanson K (1984) Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochem Biophys Res Commun 123: 291–298
Page CN (1979) Experimental aspects of fern ecology. InThe experimental biology of ferns, Dyer AF (ed.) pp 105–140. Academic Press: London
Pamukcu AM (1963) Epidemiologic studies on urinary bladder tumors in Turkish cattle. Ann N Y Acad Sci 108: 938–947
Povey AC, Evans IA, Taylor JA and O’Connor PJ (1995) Detection of DNA adducts by32P-postlabelling in mice treated with bracken extract and bracken spores. In Bracken: an environmental issue, Smith RT, Taylor JA (eds) pp95–98. International Bracken Group: Aberystwyth
Povey AC, Evans IA, Taylor JA and O’Connor PJ (1996) 32P-post-labelling analysis of DNA adducts formed in the upper gastrointestinal tissue of mice fed bracken extract or bracken spores. Br J Cancer 74: 1342–1348
Prakash AS, Pereira TN, Smith BL, Shaw G and Seawright AA (1996) Mechanism of bracken fern carcinogenesis: evidence for H- ras activation via initial adenine alkylation by ptaquiloside. Natural Toxins 4: 211–227
Simán SE and Sheffield E (2000) Polypodium vulgare plants transferred from their natural habitat to a glasshouse sporulate continuously. American Fern Journal,
Simán SE, Povey AC and Sheffield E (1999) Human health risks from fern spores? A review. Fern Gazette 15: 275–287
Smith M (1996) Spores for thought. Independent on Sunday, (Sep 22). 51
Villalobos-Salazar J, Meneses A and Salas J (1990) Carcinogenic effects in mice of milk from cows fed bracken fern Pteridium aquilinum. In Bracken biology and management. Thompson JA, Smith RT (eds) pp 247–251. Australian Institute of Agricultural Science, Occasional publication No. 40: Australia
Villalobos-Salazar J, Mora J, Meneses A and Pashov B (1995) The carcinogenic effects of bracken spores. In Bracken: an environmental issue, Smith RT, Taylor JA (eds) pp 102–103. International Bracken Group: Aberystwyth
Ward TH, Butler J, Shahbakhti H and Richards JT (1997) Comet assay studies on the activation of two diaziridinylbenzoquinones in K562 Cells. Biochem Pharmacol 53: 1115–1121
Wagner ED, Rayburn AL, Anderson D and Plewa MJ (1998) Analysis of mutagens with single cell gel electrophoresis, flow cytometry, and forward mutation assays in an isolated clone of Chinese hamster ovary cells. Environ Mol Mutagen 32: 360–368
Winston D (1998) Risk assessment of environmental exposure to fern spores MSc Thesis, The University of Manchester
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Simán, S., Povey, A., Ward, T. et al. Fern spore extracts can damage DNA. Br J Cancer 83, 69–73 (2000). https://doi.org/10.1054/bjoc.2000.1204
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DOI: https://doi.org/10.1054/bjoc.2000.1204
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