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TGFB1 gene polymorphisms: their relevance in the susceptibility to Helicobacter pylori-related diseases

Genes & Immunity volume 7, pages 640–646 (2006)Cite this article

Abstract

Recent studies have revealed elevated expression of transforming growth factor β1 (TGF-β1) in gastric mucosa of patients with gastric cancer (GC) and those undergoing ulcer repair. As production of TGF-β1 is genetically regulated, we aimed to assess whether functional polymorphisms of the TGFB1 gene are involved in susceptibility to and clinical characteristics of Helicobacter pylori-related diseases. DNA from 142 unrelated Spanish patients with GC, 200 with peptic ulcer and 342 healthy controls was typed for the MspA1I T+869C, and the Sau96I G+915C polymorphisms of the TGFB1 gene using polymerase chain reaction and RFLP analysis. H. pylori infection and CagA/VacA antibody status were determined by Western blot in patients and controls. H. pylori infection (odds ratio (OR): 11.44; 95% confidence interval (CI): 4.45–29.42; P<0.001) and non-steroidal anti-inflammatory drugs (OR: 5.07; 95% CI: 2.53–10.16; P<0.001) were identified as independent risks factors for duodenal ulcer (DU), whereas the TGFB1+869*C/C genotype was associated with reduced risk of developing the disease (OR: 0.32; 95% CI=0.15–0.68; P=0.003). Our results show that the TGFB1 T+869C gene polymorphism is involved in the susceptibility to DU and provide further evidence that host genetic factors play a key role in the pathogenesis of H. pylori-related diseases.

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Acknowledgements

This study was supported by grants from the Spanish Fondo de Investigaciones Sanitarias (Exp: 00/3035 and Exp: PI05/2739) and Instituto de Salud Carlos III (RNIHG Red C03/02).

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Authors and Affiliations

  1. Unidad Mixta de Investigación, Hospital Clínico Universitario Lozano Blesa, Zaragoza, Spain

    M A Garcia-Gonzalez

  2. Instituto Aragonés de Ciencias de la Salud, Zaragoza, Spain

    M A Garcia-Gonzalez, M Strunk, E Piazuelo, P Jiménez & A Lanas

  3. Faculty of Medicine and Department of Microbiology, Hospital Clínico Universitario Lozano Blesa, Zaragoza, Spain

    R Benito

  4. Department of Gastroenterology, Hospital San Jorge, Huesca, Spain

    S Santolaria

  5. Department of Gastroenterology, Hospital Clínico Universitario Lozano Blesa, Zaragoza, Spain

    F Sopeña, C Pascual, M A Simón, R Sainz & A Lanas

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  1. M A Garcia-Gonzalez
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Correspondence to M A Garcia-Gonzalez.

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Garcia-Gonzalez, M., Strunk, M., Piazuelo, E. et al. TGFB1 gene polymorphisms: their relevance in the susceptibility to Helicobacter pylori-related diseases. Genes Immun 7, 640–646 (2006). https://doi.org/10.1038/sj.gene.6364341

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