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Genomic structure and inducible expression of the IL-22 receptor α chain in mice

Genes & Immunity volume 4, pages 153–159 (2003)Cite this article

Abstract

IL-22 is a newly identified member of the interferon/IL-10 family. In humans, IL-22 signals through a heteroduplex receptor consisting of IL-22R and CRF2–4/IL-10Rβ. To investigate the physiological function of IL-22 and IL-22R, we isolated a cDNA encoding the mouse IL-22R, which has been a missing component of the functional receptor complex for mouse IL-22. Subsequently, we identified the genomic sequence of the mouse IL-22R gene by a database search. The gene consists of about 24 kb and is split into seven exons. Interestingly, intron 2 begins with a GC dinucleotide instead of the consensus GT, although otherwise the overall structure of the mouse IL-22R gene is strikingly similar to its human counterpart. The gene was mapped to mouse chromosome 4 in the region syntenic to the human IL-22R gene locus. In normal mice, IL-22R mRNA is detected at very low levels in restricted organs such as the kidney, liver, and lung. However, upon lipopolysaccharide stimulation, IL-22R mRNA expression is highly upregulated in the liver, in contrast to CRF2–4, which is expressed constitutively in a variety of tissues. Thus, the expression of the functional IL-22 receptor in the liver is regulated at the gene transcription level.

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References

  1. Fickenscher H, Hor S, Kupers H et al. The interleukin-10 family of cytokines. Trends Immunol 2002; 23: 89–96.

    Article  CAS  Google Scholar 

  2. Gallagher G, Dickensheets H, Eskdale J et al. Cloning, expression and initial characterization of interleukin-19 (IL- 19), a novel homologue of human interleukin-10 (IL-10). Genes Immun 2000; 1: 442–450.

    Article  CAS  Google Scholar 

  3. Blumberg H, Conklin D, Xu WF et al. Inerleukin 20: discovery, receptor identification, and role in epidermal function. Cell 2001; 104: 9–19.

    Article  CAS  Google Scholar 

  4. Dumoutier L, Louahed J, Renauld JC . Cloning and characterization of IL-10-related T cell-derived inducible factor (IL-TIF), a novel cytokine structurally related to IL-10 and inducible by IL-9. J Immunol 2000; 164: 1814–1819.

    Article  CAS  Google Scholar 

  5. Dumoutier L, Van Roost E, Ameye G, Michaux L, Renauld JC . IL-TIF/IL-22: genomic organization and mapping of the human and mouse genes. Genes Immun 2000; 1: 488–494.

    Article  CAS  Google Scholar 

  6. Jiang H, Lin JJ, Su ZZ, Goldstein NI, Fisher PB . Subtraction hybridization identifies a novel melanoma differentiation associated gene, mda-7, modulated during human melanoma differentiation, growth and progression. Oncogene 1995; 11: 2477–2486.

    CAS  Google Scholar 

  7. Knappe A, Hor S, Wittmann S, Fickenscher H . Induction of a novel cellular homolog of interleukin-10, AK155, by transformation of T lymphocytes with herpesvirus saimiri. J Virol 2000; 74: 3881–3887.

    Article  CAS  Google Scholar 

  8. Dumoutier L, Van Roost E, Colau D, Renauld JC . Human interleukin-10-related T cell-derived inducible factor: molecular cloning and functional characterization as a hepatocyte-stimulating factor. Proc Natl Acad Sci USA 2000; 97: 10144–10149.

    Article  CAS  Google Scholar 

  9. Aggarwal S, Xie MH, Maruoka M, Foster J, Gurney AL . Acinar cells of the pancreas are a target of interleukin-22. J Interferon Cytokine Res 2001; 21: 1047–1053.

    Article  CAS  Google Scholar 

  10. Xie MH, Aggarwal S, Ho WH et al. Interleukin (IL)-22, a novel human cytokine that signals through the interferon receptor-related proteins CRF2–4 and IL-22R. J Biol Chem 2000; 275: 31335–31339.

    Article  CAS  Google Scholar 

  11. Kotenko SV, Izotova LS, Mirochnitchenko OV et al. Identification of the functional interleukin-22 (IL-22) receptor complex: the IL-10R2 chain (IL-10Rbeta) is a common chain of both the IL-10 and IL-22 (IL-10-related T cell-derived inducible factor, IL-TIF) receptor complexes. J Biol Chem 2001; 276: 2725–2732.

    Article  CAS  Google Scholar 

  12. Dumoutier L, Lejeune D, Colau D, Renauld JC . Cloning and characterization of IL-22 binding protein a natural, antagonist of IL-10-related T cell-derived inducible factor/IL-22. J Immunol 2001; 166: 7090–7095.

    Article  CAS  Google Scholar 

  13. Kotenko SV, Izotova LS, Mirochnitchenko OV et al. Identification, cloning, and characterization of a novel soluble receptor that binds IL-22 and neutralizes its activity. J Immunol 2001; 166: 7096–7103.

    Article  CAS  Google Scholar 

  14. Xu W, Presnell SR, Parrish-Novak J et al. A soluble class II cytokine receptor, IL-22RA2, is a naturally occurring IL-22 antagonist. Proc Natl Acad Sci USA 2001; 98: 9511–9516.

    Article  CAS  Google Scholar 

  15. Gruenberg BH, Schoenemeyer A, Weiss B et al. A novel, soluble homologue of the human IL-10 receptor with preferential expression in placenta. Genes Immun 2001; 2: 329–334.

    Article  CAS  Google Scholar 

  16. Wang M, Tan Z, Zhang R, Kotenko SV, Liang P . Interleukin 24 (MDA-7/MOB-5) signals through two heterodimeric receptors, IL-22R1/IL-20R2 and IL-20R1/IL-20R2. J Biol Chem 2002; 277: 7341–7347.

    Article  CAS  Google Scholar 

  17. Gerhartz C, Heesel B, Sasse J et al. Differential activation of acute phase response factor/STAT3 and STAT1 via the cytoplasmic domain of the interleukin 6 signal transducer gp130, I. Definition of a novel phosphotyrosine motif mediating STAT1 activation. J Biol Chem 1996; 271: 12991–12998.

    Article  CAS  Google Scholar 

  18. Engel JD, Beug H, LaVail JH et al. cis and trans regulation of tissue-specific transcription. J Cell Sci 1992; 16 (Suppl): 21–31.

    Article  CAS  Google Scholar 

  19. Quandt K, Frech K, Karas H, Wingender E, Werner T . MatInd and MatInspector: new fast and versatile tools for detection of consensus matches in nucleotide sequence data. Nucleic Acids Res 1995; 23:4878–4884.

    Article  CAS  Google Scholar 

  20. Murai K, Murakami H, Nagata S . Myeloid-specific transcriptional activation by murine myeloid zinc- finger protein 2. Proc Natl Acad Sci USA 1998; 95: 3461–3466.

    Article  CAS  Google Scholar 

  21. Sompayrac LM, Danna KJ . Efficient infection of monkey cells with DNA of simian virus 40. Proc Natl Acad Sci USA 1981; 78: 7575–7578.

    Article  CAS  Google Scholar 

  22. Suda T, Okazaki T, Naito Y et al. Expression of the Fas ligand in cells of T cell. 20 lineage. J Immunol 1995; 154: 3806–3813.

    CAS  Google Scholar 

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Acknowledgements

We thank Ms I Hashitani for secretarial and technical assistance. This study was supported in part by the Special Coordination Funds for Promoting Science and Technology, and Grants-in-Aid for Scientific Research on Priority Areas (Diagnosis and Treatment of Cancer) from the Ministry of Education, Culture, Sports, Science and Technology of the Japanese Government.

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  1. Center for the Development of Molecular Target Drugs, Cancer Research Institute, Kanazawa University, Kanazawa, Ishikawa, Japan

    A Tachiiri, R Imamura, Y Wang, M Fukui, M Umemura & T Suda

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  1. A Tachiiri
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  2. R Imamura
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  3. Y Wang
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  4. M Fukui
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  5. M Umemura
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  6. T Suda
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Correspondence to T Suda.

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Tachiiri, A., Imamura, R., Wang, Y. et al. Genomic structure and inducible expression of the IL-22 receptor α chain in mice. Genes Immun 4, 153–159 (2003). https://doi.org/10.1038/sj.gene.6363934

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